Ontology highlight
ABSTRACT:
SUBMITTER: Mohsen MG
PROVIDER: S-EPMC6765132 | biostudies-literature | 2019 Oct
REPOSITORIES: biostudies-literature
Mohsen Michael G MG Ji Debin D Kool Eric T ET
Nucleic acids research 20191001 18
We document the preparation and properties of dimerized pentaphosphate-bridged deoxynucleotides (dicaptides) that contain reactive components of two different nucleotides simultaneously. Importantly, dicaptides are found to be considerably more stable to hydrolysis than standard dNTPs. Steady-state kinetics studies show that the dimers exhibit reasonably good efficiency with the Klenow fragment of DNA polymerase I, and we identify thermostable enzymes that process them efficiently at high temper ...[more]