Dataset Information

Effect of arabinoxylan on colonic bacterial metabolites and mucosal barrier in high-fat diet-induced rats.

ABSTRACT: The aim of this study was to demonstrate the effect of arabinoxylan on colonic mucosal barrier and metabolomic profiles in high-fat diet-induced rats. A total of 20 six-week-old male rats were arranged randomly to two groups (n = 10/group), including a high-fat diet (HFD) group and a high-fat supplemented with arabinoxylan diet (AXD) group. Results showed that feeding AXD reduced serum lipopolysaccharide in rats after 5 weeks. In colonic digesta, Escherichia coli population was reduced, while Lactobacillus, Bifidobacterium, and Bacteroidetes populations were increased in AXD group. Metabolomics assay found that the different abundances of 84 metabolites were observed, involving lipid, carbohydrate, and nitrogenous metabolism in colonic digesta. In colonic mucosa, AXD up-regulated gene level of tight-junction-related proteins. Meanwhile, lower TNF-? and IL-1? levels were related to TLR4/NF-?B/MyD88 pathway in AXD group. In conclusion, arabinoxylan could change colonic microbial metabolism and improve the colonic mucosal barrier via modulating intestinal microflora and tight junction proteins.

SUBMITTER: Li S

PROVIDER: S-EPMC6766541 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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Effect of arabinoxylan on colonic bacterial metabolites and mucosal barrier in high-fat diet-induced rats.

Li Shanshan S Sun Yanan Y Hu Xinxin X Qin Wen W Li Cheng C Liu Yuntao Y Liu Aiping A Zhao Ye Y Wu Dingtao D Lin Derong D Zhang Qing Q Chen Daiwen D Chen Hong H

Food science & nutrition 20190812 9

The aim of this study was to demonstrate the effect of arabinoxylan on colonic mucosal barrier and metabolomic profiles in high-fat diet-induced rats. A total of 20 six-week-old male rats were arranged randomly to two groups (<i>n</i> = 10/group), including a high-fat diet (HFD) group and a high-fat supplemented with arabinoxylan diet (AXD) group. Results showed that feeding AXD reduced serum lipopolysaccharide in rats after 5 weeks. In colonic digesta, <i>Escherichia coli</i> population was red ...[more]

PMID: 31572598

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Project description:Increased consumption of energy-rich foods is a key factor in overweight, obesity, and associated metabolic disorders. This would be, at least in part, related to microbiota disturbance. In rodent models of obesity, microbiota disruption has been associated with alteration of the intestinal barrier, endotoxemia, inflammation grade, and insulin sensitivity. The aim of the present study was to assess the effects of a high-fat diet (HFD), fed at two energetic levels, on microbiota, intestinal barrier, and inflammatory and metabolic parameters in dogs. A HFD (33% fat as fed, 4,830 kcal/kg) was given to 24 healthy Beagle dogs at 100% (HF-100; n = 8) and at 150% (HF-150; n = 16) of their maintenance energy requirements for 8 weeks. Analysis of similarity revealed a significant difference in gut microbiota β-diversity following the diet compared to week 0 in both groups while α-diversity was lower only in the HF-150 group. Firmicutes/Bacteroidetes ratio was higher in the HF-150 group compared to the HF-100 group at weeks 2 and 8. A reduction in insulin sensitivity was observed over time in the HF150 group. Neither endotoxemia nor inflammation was observed in either group, did not find supporting data for the hypothesis that the microbiota is involved in the decline of insulin sensitivity through metabolic endotoxemia and low-grade inflammation. Colonic permeability was increased at week 4 in both groups and returned to initial levels at week 8, and was associated with modifications to the expression of genes involved in colonic barrier function. The increase in intestinal permeability may have been caused by the altered intestinal microbiota and increased expression of genes encoding tight junction proteins might indicate a compensatory mechanism to restore normal permeability. Although simultaneous changes to the microbiota, barrier permeability, inflammatory, and metabolic status have not been observed, such a causal link cannot be excluded in dogs overfed on a HFD. Further studies are necessary to better understand the link between HFD, intestinal microbiota and the host.

Dataset Information

Effect of arabinoxylan on colonic bacterial metabolites and mucosal barrier in high-fat diet-induced rats.

Publications

Effect of arabinoxylan on colonic bacterial metabolites and mucosal barrier in high-fat diet-induced rats.

Similar Datasets

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