Dataset Information

Novel Y-Chromosome Long Non-Coding RNAs Expressed in Human Male CNS During Early Development.

ABSTRACT: Global microarray gene expression analyses previously demonstrated differences in female and male embryos during neurodevelopment. In particular, before sexual maturation of the gonads, the differences seem to concentrate on the expression of genes encoded on the X- and Y-chromosomes. To investigate genome-wide differences in expression during this early developmental window, we combined high-resolution RNA sequencing with qPCR to analyze brain samples from human embryos during the first trimester of development. Our analysis was tailored for maximum sensitivity to discover Y-chromosome gene expression, but at the same time, it was underpowered to detect X-inactivation escapees. Using this approach, we found that 5 out of 13 expressed gametolog pairs showed unbalanced gene dosage, and as a consequence, a male-biased expression. In addition, we found six novel non-annotated long non-coding RNAs on the Y-chromosome with conserved expression patterns in newborn chimpanzee. The tissue specific and time-restricted expression of these long non-coding RNAs strongly suggests important functions during central nervous system development in human males.

SUBMITTER: Johansson MM

PROVIDER: S-EPMC6769107 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Novel Y-Chromosome Long Non-Coding RNAs Expressed in Human Male CNS During Early Development.

Johansson Martin M MM Pottmeier Philipp P Suciu Pascalina P Ahmad Tauseef T Zaghlool Ammar A Halvardson Jonatan J Darj Elisabeth E Feuk Lars L Peuckert Christiane C Jazin Elena E

Frontiers in genetics 20190924

Global microarray gene expression analyses previously demonstrated differences in female and male embryos during neurodevelopment. In particular, before sexual maturation of the gonads, the differences seem to concentrate on the expression of genes encoded on the X- and Y-chromosomes. To investigate genome-wide differences in expression during this early developmental window, we combined high-resolution RNA sequencing with qPCR to analyze brain samples from human embryos during the first trimest ...[more]

PMID: 31608120

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Dataset Information

Novel Y-Chromosome Long Non-Coding RNAs Expressed in Human Male CNS During Early Development.

Publications

Novel Y-Chromosome Long Non-Coding RNAs Expressed in Human Male CNS During Early Development.

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