Dataset Information

Analysis of the colonic mucosa associated microbiota (MAM) using brushing samples during colonic endoscopic procedures.

ABSTRACT: The mucosa-associated microbiota is an important component in human microbiota. The aim was to investigate mucosa-associated microbiota using brush samples during endoscopic procedures and compare with fecal microbiota. Seven patients who were planning to undergo a routine colonoscopy were recruited. Mucosal brushing samples were taken from 3 sites (terminal ileum, ascending and sigmoid colon), and a fecal sample was taken on the morning of colonoscopy. The samples were immediately placed in microcentrifuge tubes containing DNA stabilization reagent and analyzed using the next generation sequencer. The individual differences in microbiota were more evident than the differences of the sampling sites. Actinobacteria was more abundant and Bacteroidetes was less in the brush samples than those in the fecal samples. Taxonomic composition at the genus level and the proportion of genes responsible for some functions in the brushing samples tended to be different from those in the fecal samples. Bulleidia and Oribacteriumi were significantly more abundant and the proportions of genes responsible for transcription factors and phosphotransferase system were higher in ileal mucous than those in fecal samples. Brushing during colonoscopic procedure instead of using feces samples might be useful to analyze mucosa-associated microbiota.

SUBMITTER: Matsumoto H

PROVIDER: S-EPMC6769413 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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Analysis of the colonic mucosa associated microbiota (MAM) using brushing samples during colonic endoscopic procedures.

Matsumoto Hiroshi H Kuroki Yasutoshi Y Higashi Seiya S Goda Kyosuke K Fukushima Shinya S Katsumoto Ryo R Oosawa Motoyasu M Murao Takahisa T Ishii Manabu M Oka Kentaro K Takahashi Motomichi M Osaki Takako T Kamiya Shigeru S Shiotani Akiko A

Journal of clinical biochemistry and nutrition 20190727 2

The mucosa-associated microbiota is an important component in human microbiota. The aim was to investigate mucosa-associated microbiota using brush samples during endoscopic procedures and compare with fecal microbiota. Seven patients who were planning to undergo a routine colonoscopy were recruited. Mucosal brushing samples were taken from 3 sites (terminal ileum, ascending and sigmoid colon), and a fecal sample was taken on the morning of colonoscopy. The samples were immediately placed in mic ...[more]

PMID: 31592051

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Dataset Information

Analysis of the colonic mucosa associated microbiota (MAM) using brushing samples during colonic endoscopic procedures.

Publications

Analysis of the colonic mucosa associated microbiota (MAM) using brushing samples during colonic endoscopic procedures.

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