Dataset Information

Vitamin D Attenuates Oxidative Damage and Inflammation in Retinal Pigment Epithelial Cells.

ABSTRACT: Age-related macular degeneration (AMD), the most common visual disorder in elderly people, is characterized by the formation of deposits beneath the retinal pigment epithelium (RPE) and by dysfunction of RPE and photoreceptor cells. The biologically active form of vitamin D, 1,25-(OH)2D3 (VITD), is categorized as a multifunctional steroid hormone that modulates many transcriptional processes of different genes and is involved in a broad range of cellular functions. Epidemiological and genetic association studies demonstrate that VITD may have a protective role in AMD, while single nucleotide polymorphisms in the vitamin D metabolism gene (CYP24A1) increase the risk of AMD. However, the functional mechanisms of VITD in AMD are not fully understood. In the current study, we investigated the impact of VITD on H₂O₂-induced oxidative stress and inflammation in human RPE cells. We demonstrate that exposure to H₂O₂ caused significantly reduced cell viability, increased production of reactive oxygen species (ROS), lowered expression of antioxidant enzymes and enhanced inflammation. VITD exposure notably counteracted the above H₂O₂-induced effects. Our data suggest that VITD protects the RPE from oxidative damage and elucidate molecular mechanisms of VITD deficiency in the development of AMD.

SUBMITTER: Tohari AM

PROVIDER: S-EPMC6770403 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature

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Vitamin D Attenuates Oxidative Damage and Inflammation in Retinal Pigment Epithelial Cells.

Tohari Ali Mohammad AM Alhasani Reem Hasaballah RH Biswas Lincoln L Patnaik Sarita Rani SR Reilly James J Zeng Zhihong Z Shu Xinhua X

Antioxidants (Basel, Switzerland) 20190824 9

Age-related macular degeneration (AMD), the most common visual disorder in elderly people, is characterized by the formation of deposits beneath the retinal pigment epithelium (RPE) and by dysfunction of RPE and photoreceptor cells. The biologically active form of vitamin D, 1,25-(OH)2D3 (VITD), is categorized as a multifunctional steroid hormone that modulates many transcriptional processes of different genes and is involved in a broad range of cellular functions. Epidemiological and genetic as ...[more]

PMID: 31450606

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Dataset Information

Vitamin D Attenuates Oxidative Damage and Inflammation in Retinal Pigment Epithelial Cells.

Publications

Vitamin D Attenuates Oxidative Damage and Inflammation in Retinal Pigment Epithelial Cells.

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