Unknown

Dataset Information

0

Squaramate-Modified Nucleotides and DNA for Specific Cross-Linking with Lysine-Containing Peptides and Proteins.


ABSTRACT: Squaramate-linked 2'-deoxycytidine 5'-O-triphosphate was synthesized and found to be good substrate for KOD XL DNA polymerase in primer extension or PCR synthesis of modified DNA. The resulting squaramate-linked DNA reacts with primary amines to form a stable diamide linkage. This reaction was used for bioconjugations of DNA with Cy5 and Lys-containing peptides. Squaramate-linked DNA formed covalent cross-links with histone proteins. This reactive nucleotide has potential for other bioconjugations of nucleic acids with amines, peptides or proteins without need of any external reagent.

SUBMITTER: Ivancova I 

PROVIDER: S-EPMC6771961 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC8362068 | biostudies-literature
| S-EPMC3982193 | biostudies-literature
| S-EPMC4650688 | biostudies-literature
| S-EPMC6682051 | biostudies-literature
| S-EPMC7154546 | biostudies-literature
| S-EPMC6310614 | biostudies-literature
| S-EPMC4955596 | biostudies-literature
| S-EPMC2887952 | biostudies-literature
| S-EPMC2568988 | biostudies-literature
| S-EPMC6491775 | biostudies-literature