Ontology highlight
ABSTRACT:
SUBMITTER: Dunbar KL
PROVIDER: S-EPMC6772006 | biostudies-literature | 2019 Sep
REPOSITORIES: biostudies-literature
Angewandte Chemie (International ed. in English) 20190807 37
Thioamide-containing nonribosomal peptides (NRPs) are exceedingly rare. Recently the biosynthetic gene cluster for the thioamidated NRP antibiotic closthioamide (CTA) was reported, however, the enzyme responsible for and the timing of thioamide formation remained enigmatic. Here, genome editing, biochemical assays, and mutational studies are used to demonstrate that an Fe-S cluster containing member of the adenine nucleotide α-hydrolase protein superfamily (CtaC) is responsible for sulfur incorp ...[more]