Platelet-derived extracellular vesicles released after trauma promote hemostasis and contribute to DVT in mice.

ABSTRACT:

Background

Traumatic injury can lead to dysregulation of the normal clotting system, resulting in hemorrhagic and thrombotic complications. Platelet activation is robust following traumatic injury and one process of platelet activation is to release of extracellular vesicles (PEV) that carry heterogenous cargo loads and surface ligands.

Objectives

We sought to investigate and characterize the release and function of PEVs generated following traumatic injury.

Methods

PEV content and quantity in circulation following trauma in humans and mice was measured using flow cytometry, size exclusion chromatography, and nanoparticle tracking analysis. PEVs were isolated from circulation and the effects on thrombin generation, bleeding time, hemorrhage control, and thrombus forma

SUBMITTER: Dyer MR 

PROVIDER: S-EPMC6773503 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature