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Saliva-derived cfDNA is applicable for EGFR mutation detection but not for quantitation analysis in non-small cell lung cancer.


ABSTRACT: BACKGROUND:Both quantitative and qualitative aspects of plasma cell-free DNA (plasma cfDNA, pcfDNA) have been well-studied as potential biomarkers in non-small cell lung cancer (NSCLC). Accumulating evidence has proven that saliva also has the potential for the detection and analysis of circulating free DNA (saliva cfDNA, scfDNA). METHODS:In the current study, we aimed to explore the potential application of scfDNA in NSCLC diagnostics and consistency of epidermal growth factor receptor (EGFR) mutation detection in paired pcfDNA and scfDNA using droplet digital PCR (ddPCR) and analyze the relationship between EGFR mutations and clinical treatment response. RESULTS:In the quantitative cohort study, scfDNA concentration in NSCLC patients was no different from that in healthy donors, or in benign patients. ScfDNA concentration was significantly lower than pcfDNA concentration, yet they were not statistically significant in relevance (Spearman's rank correlation r = -0.123, P =?0.269). In the qualitative cohort study, the overall concordance rate of EGFR mutations between pcfDNA and scfDNA was 83.78% (31 of 37; k = 0.602; P

SUBMITTER: Ding S 

PROVIDER: S-EPMC6775000 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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Saliva-derived cfDNA is applicable for EGFR mutation detection but not for quantitation analysis in non-small cell lung cancer.

Ding Shanshan S   Song Xingguo X   Geng Xinran X   Liu Lele L   Ma Hongxin H   Wang Xiujuan X   Wei Ling L   Xie Li L   Song Xianrang X  

Thoracic cancer 20190823 10


<h4>Background</h4>Both quantitative and qualitative aspects of plasma cell-free DNA (plasma cfDNA, pcfDNA) have been well-studied as potential biomarkers in non-small cell lung cancer (NSCLC). Accumulating evidence has proven that saliva also has the potential for the detection and analysis of circulating free DNA (saliva cfDNA, scfDNA).<h4>Methods</h4>In the current study, we aimed to explore the potential application of scfDNA in NSCLC diagnostics and consistency of epidermal growth factor re  ...[more]

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2024-06-20 | GSE269746 | GEO