Variation of ¹³C and ¹⁵N enrichments in different plant components of labeled winter wheat (Triticum aestivum L.).

ABSTRACT: Information on the homogeneity and distribution of ¹³carbon (¹³C) and nitrogen (¹⁵N) labeling in winter wheat (Triticum aestivum L.) is limited. We conducted a dual labeling experiment to evaluate the variability of ¹³C and ¹⁵N enrichment in aboveground parts of labeled winter wheat plants. Labeling with ¹³C and ¹⁵N was performed on non-nitrogen fertilized (-N) and nitrogen fertilized (+N, 250 kg N ha^-1) plants at the elongation and grain filling stages. Aboveground parts of wheat were destructively sampled at 28 days after labeling. As winter wheat growth progressed, ? ¹³C values of wheat ears increased significantly, whereas those of leaves and stems decreased significantly. At the elongation stage, N addition tended to reduce the aboveground ? ¹³C values through dilution of C uptake. At the two stages, upper (newly developed) leaves were more highly enriched with ¹³C compared with that of lower (aged) leaves. Variability between individual wheat plants and among pots at the grain filling stage was smaller than that at the elongation stage, especially for the -N treatment. Compared with those of ¹³C labeling, differences in ¹⁵N excess between aboveground components (leaves and stems) under ¹⁵N labeling conditions were much smaller. We conclude that non-N fertilization and labeling at the grain filling stage may produce more uniformly ¹³C-labeled wheat materials, whereas the materials were more highly ¹³C-enriched at the elongation stage, although the ? ¹³C values were more variable. The ¹⁵N-enriched straw tissues via urea fertilization were more uniformly labeled at the grain filling stage compared with that at the elongation stage.

SUBMITTER: Sun Z 

PROVIDER: S-EPMC6778429 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature