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Destabilization of the Tumor-Inducing Plasmid from an Octopine-Type Agrobacterium tumefaciens Lineage Drives a Large Deletion in the Co-resident At Megaplasmid.


ABSTRACT: Bacteria with multi-replicon genome organizations, including members of the family Rhizobiaceae, often carry a variety of niche-associated functions on large plasmids. While evidence exists for cross-replicon interactions and co-evolution between replicons in many of these systems, remarkable strain-to-strain variation is also observed for extrachromosomal elements, suggesting increased genetic plasticity. Here, we show that curing of the tumor-inducing virulence plasmid (pTi) of an octopine-type Agrobacterium tumefaciens lineage leads to a large deletion in the co-resident At megaplasmid (pAt). The deletion event is mediated by a repetitive IS-element, IS66, and results in a variety of environment-dependent fitness consequences, including loss of independent conjugal transfer of the plasmid. Interestingly, a related and otherwise wild-type A. tumefaciens strain is missing exactly the same large pAt segment as the pAt deletion derivatives, suggesting a similar event over its natural history. Overall, the findings presented here uncover a novel genetic interaction between the two large plasmids of A. tumefaciens and provide evidence for cross-replicon integration and co-evolution of these plasmids.

SUBMITTER: Barton IS 

PROVIDER: S-EPMC6778807 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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Destabilization of the Tumor-Inducing Plasmid from an Octopine-Type <i>Agrobacterium tumefaciens</i> Lineage Drives a Large Deletion in the Co-resident At Megaplasmid.

Barton Ian S IS   Platt Thomas G TG   Rusch Douglas B DB   Fuqua Clay C  

G3 (Bethesda, Md.) 20191007 10


Bacteria with multi-replicon genome organizations, including members of the family <i>Rhizobiaceae</i>, often carry a variety of niche-associated functions on large plasmids. While evidence exists for cross-replicon interactions and co-evolution between replicons in many of these systems, remarkable strain-to-strain variation is also observed for extrachromosomal elements, suggesting increased genetic plasticity. Here, we show that curing of the tumor-inducing virulence plasmid (pTi) of an octop  ...[more]

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