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[Small interfering RNA-mediated ?-enolase knockdown suppresses glycolysis and proliferation of human glioma U251 cells in vitro].


ABSTRACT: OBJECTIVE:To investigate the role of ?-enolase (ENO1) in regulating glucose metabolism and cell growth in human glioma cells. METHODS:Glucose uptake and lactate generation were assessed to evaluate the changes in glucose metabolism in human glioma U251 cells with small interfering RNA (siRNA)-mediated ENO1 knockdown. MTT assay and 5-ethynyl-2'-deoxyuridine (EdU) staining were used to examine the cell growth and cell cycle changes following siRNA transfection of the cells. RESULTS:Transfection of U251 cells with siRNA-ENO1 markedly reduced glucose uptake (P=0.023) and lactate generation (P=0.007) in the cells and resulted in significant suppression of cell proliferation (*P<0.05) since the second day following the transfection. Transfection with siRNA-ENO1 also obviously suppressed cell cycle G1/S transition in the cells (P=0.0425). The expressions of HK2 and LDHA, the marker genes for glucose metabolism, were significantly down-regulated in the cells with siRNA-mediated ENO1 knockdown. CONCLUSION:ENO1 as a potential oncogene promotes glioma cell growth by positively modulating glucose metabolism.

SUBMITTER: Luo QS 

PROVIDER: S-EPMC6779634 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature

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[Small interfering RNA-mediated α-enolase knockdown suppresses glycolysis and proliferation of human glioma U251 cells in vitro].

Luo Qi-Sheng QS   Fu Huang-de HD   Huang Hai-Neng HN   Huang Hua-Dong HD   Luo Kun-Xiang KX   Li Chuan-Yu CY   Qin Cheng-Jian CJ   Li Xue-Yu XY   Luo Hong-Cheng HC   Wang Jun-Li JL   Tang Qian-Li QL  

Nan fang yi ke da xue xue bao = Journal of Southern Medical University 20171101 11


<h4>Objective</h4>To investigate the role of α-enolase (ENO1) in regulating glucose metabolism and cell growth in human glioma cells.<h4>Methods</h4>Glucose uptake and lactate generation were assessed to evaluate the changes in glucose metabolism in human glioma U251 cells with small interfering RNA (siRNA)-mediated ENO1 knockdown. MTT assay and 5-ethynyl-2'-deoxyuridine (EdU) staining were used to examine the cell growth and cell cycle changes following siRNA transfection of the cells.<h4>Resul  ...[more]

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