Dataset Information

Characterization of Beta-Lactamases in Bloodstream-Infection Escherichia coli: Dissemination of bla_ADC _- ₁₆₂ and bla_CMY- ₂ Among Bacteria via an IncF Plasmid.

ABSTRACT:

Objectives

To describe the molecular characteristics of beta-lactamases in bloodstream-infection Escherichia coli isolated from elderly patients, and to determine the genotypic patterns of bla _CMY _- ₂ and bla _ADC _- ₁₆₂.

Methods

A total of 50 bloodstream-infection E. coli isolates were obtained from patients aged > 50 years at Shanghai Sixth People's Hospital South Campus during 2015-2018. The isolates were subjected to beta-lactamase detection using phenotypic and molecular methods. Beta-lactamase genes were verified by sequencing and the phylogenetic relationships of the isolates were analyzed by multilocus sequence typing (MLST). The transferability of plasmids carrying bla _CMY- ₂ and bla _ADC- ₁₆₂ genes was verified by conjugation experiments and plasmid replicon typing.

Results

Eight beta-lactamase subtypes were detected in 50 isolates of bloodstream-infection E. coli. bla _TEM- ₁ (21/50) was the most common beta-lactamase gene, followed by bla _CTX-M- ₁₄ (8/50), bla _OXA- ₂₇ (5/50), bla _CTX-M- ₂₇ (3/50), bla _CTX-M- ₆₅ (1/50), bla _ADC- ₁₆₂ (1/50), and bla _CMY- ₂ (1/50). Of these, bla _ADC- ₁₆₂ (ST95-A), and bla _CMY- ₂ (ST95-B2) have not previously been reported in bloodstream-infection E. coli. In 21 isolates, beta-lactamase genes were located on conjugative plasmids belonging to incompatibility groups FrepB (n = 7), FIA (n = 1), FIC (n = 2), K (n = 8), N (n = 1), and I (n = 1), and bla _CTX-M was associated with the common elements ISEcp1, IS903, and IS26, but with special sequences (region V, region Y, and region W) for ISEcp1 in 14 isolates.

Conclusion

To the best of our knowledge, this study provides the first molecular characterization of beta-lactamase genes in E. coli isolated from the bloodstream in elderly patients. Beta-lactamase genes were detected at a relatively high frequency in elderly patients with bloodstream E. coli infections. Plasmid replicon analysis showed that horizontal dissemination of beta-lactamase genes was mainly mediated by IncK and IncF plasmids, which could encode multidrug resistance genes. The study also provides the first report of ISAba1-bla _ADC _- ₁₆₂-tnpA and ISEcp1-bla _CTX-M- ₁₄-IS903-bla _CMY- ₂-blc-sugE in E. coli, and demonstrates IncF plasmid-mediated bla _ADC _- ₁₆₂ and bla _CMY- ₂ gene dissemination among bacteria.

SUBMITTER: Xiao L

PROVIDER: S-EPMC6781614 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Publications

Characterization of Beta-Lactamases in Bloodstream-Infection Escherichia coli: Dissemination of blaADC - 162 and blaCMY- 2 Among Bacteria via an IncF Plasmid.

Xiao Linlin L Wang Xiaotong X Kong Nana N Zhang Long L Cao Mei M Sun Muzhen M Wei Quhao Q Liu Weiwei W

Frontiers in microbiology 20191001

<h4>Objectives</h4>To describe the molecular characteristics of beta-lactamases in bloodstream-infection Escherichia coli isolated from elderly patients, and to determine the genotypic patterns of bla CMY - 2 and bla ADC - 162.<h4>Methods</h4>A total of 50 bloodstream-infection E. coli isolates were obtained from patients aged > 50 years at Shanghai Sixth People's Hospital South Campus during 2015-2018. ...[more]

PMID: 31632358

Similar Datasets

Project description:Escherichia coli sequence type 131 (ST131) has contributed to the spread of extended-spectrum beta-lactamase (ESBL) and has emerged as the dominant cause of hospital- and community-acquired urinary tract infections. Here, we report for the first time an in-depth analysis of whole-genome sequencing (WGS) of 4 ESBL-producing E. coli ST131 isolates recovered from patients in two hospitals in Armenia using Illumina short-read sequencing for accurate base calling to determine their genotype and to infer their phylogeny and using Oxford Nanopore Technologies long-read sequencing to resolve plasmid and chromosomal genetic elements. Genotypically, the four Armenian isolates were identified as part of the H30Rx/clade C2 (n = 2) and H41/clade A (n = 2) lineages and were phylogenetically closely related to isolates from the European Nucleotide Archive (ENA) database previously recovered from patients in the United States, Australia, and New Zealand. The Armenian isolates recovered in this study had chromosomal integration of the blaCTX-M-15 gene in the H30Rx isolates and a high number of virulence genes found in the H41 isolates associated with the carriage of a rare genomic island (in the context of E. coli ST131) containing the S fimbrial, salmochelin siderophore, and microcin H47 virulence genes. Furthermore, our data show the evolution of the IncF[2:A2:B20] plasmid harboring both blaCTX-M-15 and blaCTX-M-27 genes, derived from the recombination of genes from an IncF[F2:A-:B-] blaCTX-M-15-associated plasmid into the IncF[F1:A2:B20] blaCTX-M-27-associated plasmid backbone seen in two genetically closely related H41 Armenian isolates. IMPORTANCE Combining short and long reads from whole-genome sequencing analysis provided a genetic context for uncommon genes of clinical importance to better understand transmission and evolutionary features of ESBL-producing uropathogenic E. coli (UPEC) ST131 isolates recovered in Armenia. Using hybrid genome assembly in countries lacking genomic surveillance studies can inform us about new lineages not seen in other countries with genes encoding high virulence and antibiotic resistance harbored on mobile genetic elements.