Characterization of Beta-Lactamases in Bloodstream-Infection Escherichia coli: Dissemination of bla_{ADC - 162} and bla_{CMY- 2} Among Bacteria via an IncF Plasmid.

ABSTRACT:

Objectives

To describe the molecular characteristics of beta-lactamases in bloodstream-infection Escherichia coli isolated from elderly patients, and to determine the genotypic patterns of bla _{CMY - 2} and bla _{ADC - 162}.

Methods

A total of 50 bloodstream-infection E. coli isolates were obtained from patients aged > 50 years at Shanghai Sixth People's Hospital South Campus during 2015-2018. The isolates were subjected to beta-lactamase detection using phenotypic and molecular methods. Beta-lactamase genes were verified by sequencing and the phylogenetic relationships of the isolates were analyzed by multilocus sequence typing (MLST). The transferability of plasmids carrying bla

SUBMITTER: Xiao L 

PROVIDER: S-EPMC6781614 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature