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Ubiquitin ligase RNF5 serves an important role in the development of human glioma.

ABSTRACT: The ubiquitin ligase ring finger protein 5 (RNF5) has previously been associated with the development of breast cancer. Patients with breast cancer and high RNF5 expression have been demonstrated to have a shorter survival time compared with patients with low RNF5 expression. However, the role of RNF5 in human glioma has not been determined. The present study analyzed the role of RNF5 in gliomas using bioinformatics analysis. The results revealed that RNF5 was differentially expressed in non-cancerous brain tissues and different grades of glioma. Furthermore, a high RNF5 expression in patients with glioma was associated with an improved prognosis compared with patients with low expression. Gene Set Enrichment Analysis revealed that RNF5 was particularly associated with 'Wnt signaling pathway', 'apoptosis', 'focal adhesion' and 'cytokine-cytokine receptor interaction' in patients with glioma. Additionally, 4 potential ubiquitination substrates for RNF5 were predicted, including sorting nexin 10, proprotein convertase subtilisin/kexin type 1, leucine rich glioma inactivated 1 and solute carrier family 39 member 12. These findings provided the basis for further investigation on the role of RNF5 in tumors.

SUBMITTER: Gao Y

PROVIDER: S-EPMC6781729 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature

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Ubiquitin ligase RNF5 serves an important role in the development of human glioma.

Gao Yong Y Xuan Chengmin C Jin Mingwei M An Qi Q Zhuo Baobiao B Chen Xincheng X Wang Lei L Wang Yuan Y Sun Qingzeng Q Shi Yingchun Y

Oncology letters 20190904 5

The ubiquitin ligase ring finger protein 5 (RNF5) has previously been associated with the development of breast cancer. Patients with breast cancer and high RNF5 expression have been demonstrated to have a shorter survival time compared with patients with low RNF5 expression. However, the role of RNF5 in human glioma has not been determined. The present study analyzed the role of RNF5 in gliomas using bioinformatics analysis. The results revealed that RNF5 was differentially ...[more]

PMID: 31611975

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Project description:Periodontal accelerate osteogenesis orthodontics (PAOO) is an extension of described techniques that surgically alter the alveolar bone; however, the specific mechanism underlying the technique is not completely understood. The aim of the present study was to evaluate the roles of microRNA (miR)?21 during PAOO. Sprague?Dawley rats were divided into the following four groups: i) Group tooth movement (TM), underwent TM and were administered normal saline (NS); ii) Group PAOO, underwent PAOO + TM and were administered NS; iii) Group agomiR?21, underwent PAOO + TM and were administered agomiR?21; and iv) Group antagomiR?21, underwent PAOO + TM and were administered antagomiR?21. To validate the rat model of PAOO, morphological analyses were performed and measurements were collected. Reverse transcription?quantitative PCR, western blotting and immunohistochemical staining were performed to examine the expression levels of programmed cell death 4 (PDCD4), activin A receptor type 2B (ACVR2b), receptor activator of NF??? ligand (RANKL) and C?Fos. Dual?luciferase reporter assays were performed to validate PDCD4 as a target of miR?21 in vitro. Following 7 days of treatment, the TM distance of group PAOO was longer compared with groups TM and antagomiR?21 (P<0.05), but shorter compared with group agomiR?21 (P<0.05). Tartrate?resistant acid phosphatase staining indicated that following treatment with agomiR?21, osteoclast activity was notably increased, whereas the mRNA and protein expression levels of PDCD4 were notably decreased compared with group PAOO. The mRNA and protein expression levels of RANKL and C?Fos in group agomiR?21 were notably increased compared with group PAOO, whereas group antagomiR?21 displayed the opposite pattern (P<0.05). With regard to ACVR2b, no significant differences were observed among the group agomiR?21 and antagomiR?21 compared with group PAOO. Bioinformatics analysis predicted that PDCD4 was a potential target gene of miR?21, and dual?luciferase reporter assays demonstrated that miR?21 directly targeted PDCD4. In conclusion, the present study demonstrated that miR?21 serves an important role during PAOO?mediated orthodontic TM.

Dataset Information

Ubiquitin ligase RNF5 serves an important role in the development of human glioma.

Publications

Ubiquitin ligase <i>RNF5</i> serves an important role in the development of human glioma.

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