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Song-induced phosphorylation of cAMP response element-binding protein in the songbird brain.

ABSTRACT: We have investigated the participation of cAMP response element-binding protein (CREB) in the response of the songbird brain to a natural auditory stimulus, a conspecific song. The cells in the two song control nuclei, the higher vocal center (HVC) and area X of zebra finches (Taeniopygia guttata), were intensely stained with an anti-CREB monoclonal antibody. Double-labeling studies showed that CREB immunoreactivity was detected only in area X-projecting neurons in the HVC. The cloned CREB cDNA from zebra finches (zCREB) is highly homologous to mammalian delta CREB. Phosphorylation of zCREB at Ser119 in area X-projecting HVC neurons was induced by hearing tape-recorded conspecific songs of zebra finches, but not by birdsongs of another species or white noise. These results raise the possibility that zCREB plays a crucial role in the sensory process of song learning.

SUBMITTER: Sakaguchi H

PROVIDER: S-EPMC6782717 | biostudies-literature | 1999 May

REPOSITORIES: biostudies-literature

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Song-induced phosphorylation of cAMP response element-binding protein in the songbird brain.

Sakaguchi H H Wada K K Maekawa M M Watsuji T T Hagiwara M M

The Journal of neuroscience : the official journal of the Society for Neuroscience 19990501 10

We have investigated the participation of cAMP response element-binding protein (CREB) in the response of the songbird brain to a natural auditory stimulus, a conspecific song. The cells in the two song control nuclei, the higher vocal center (HVC) and area X of zebra finches (Taeniopygia guttata), were intensely stained with an anti-CREB monoclonal antibody. Double-labeling studies showed that CREB immunoreactivity was detected only in area X-projecting neurons in the HVC. The cloned CREB cDNA ...[more]

PMID: 10234027

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