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The association of speckle-type POZ protein with lymph node metastasis and prognosis in cancer patients: A meta-analysis.

ABSTRACT: BACKGROUND:Speckle-type POZ protein (SPOP) has recently been reported as a prognostic tumor biomarker. However, the predictive value of SPOP remains controversial in human cancers. The current meta-analysis was performed to obtain a comprehensive evaluation of the relationship between SPOP expression and prognosis of cancer patients. METHODS:Embase, Pubmed, Web of Science, and Chinese Biomedical Literature database were systematically searched up to January 2, 2019. The pooled hazard ratios (HRs) and/or pooled odds ratios (ORs) with 95% confidence intervals (CIs) were used to quantitatively assess the relationship of SPOP expression with prognosis and lymph node metastasis (LNM). RESULTS:A total of 9 studies with 928 patients were included in this meta-analysis. The results showed that low SPOP expression was significantly related to poor overall survival (high/low: HR?=?0.55; 95% CI: 0.38-0.79, P?=?.001), especially for digestive system cancers (high/low: HR?=?0.46; 95% CI: 0.27-0.78, P?=?.003). However, SPOP expression did not affect progression-free survival in cancer patients (high/low: HR?=?2.07; 95% CI: 0.16-26.70, P?=?.578). Additionally, the association between SPOP overexpression and LNM was positive in patients with clear cell renal cell carcinoma (ccRCC) (OR?=?5.26; 95% CI: 1.66-16.68, P?=?.005) but negative in cancer patients without ccRCC (OR?=?0.36; 95% CI: 0.21-0.62, P?

SUBMITTER: Cheng F

PROVIDER: S-EPMC6783248 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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The association of speckle-type POZ protein with lymph node metastasis and prognosis in cancer patients: A meta-analysis.

Cheng Fei F Zeng Chunyan C Zeng Ling L Wu Chayan C Chen Youxiang Y

Medicine 20191001 40

<h4>Background</h4>Speckle-type POZ protein (SPOP) has recently been reported as a prognostic tumor biomarker. However, the predictive value of SPOP remains controversial in human cancers. The current meta-analysis was performed to obtain a comprehensive evaluation of the relationship between SPOP expression and prognosis of cancer patients.<h4>Methods</h4>Embase, Pubmed, Web of Science, and Chinese Biomedical Literature database were systematically searched up to January 2, 2019. The pooled haz ...[more]

PMID: 31577764

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Project description:BackgroundSpeckle-type POZ protein(SPOP), a substrate adaptor of Cul3 ubiquitin ligase, plays crucial roles in solid neoplasms by promoting the ubiquitination and degradation of substrates. Limited studies have shown that SPOP is overexpressed in human renal cell carcinoma (RCC) tissue. However, the exact role of SPOP in RCC remains unclear and needs to be further elucidated. The present study showed that SPOP was expressed at different levels in different RCC cell lines. The purpose of this study was to explore the roles of SPOP in the biological features of RCC cells and the expression levels of SPOP in human tissue microarray (TMA) and kidney tissues.MethodsHere, SPOP was overexpressed by lentiviral vector transfection in ACHN and Caki-1 cells, and SPOP was knocked down in Caki-2 cells with similar transfection methods. The transfection efficiency was evaluated by quantitative PCR and western blotting analyses. The role of SPOP in the proliferation, migration, invasion and apoptosis of cell lines was determined by the MTT, wound-healing, transwell and flow cytometry assays. Moreover, the cells were treated with different drug concentrations in proliferation and apoptosis assays to investigate the effect of sunitinib and IFN-α2b on the proliferation and apoptosis of SPOP-overexpressing cells and SPOP-knockdown RCC cells. Finally, immunohistochemical staining of SPOP was performed in kidney tissues and TMAs, which included RCC tissues and corresponding adjacent normal tissues.ResultsOverexpression of SPOP inhibited cell proliferation, migration and invasion and increased cell apoptosis. Interestingly, sunitinib and IFN-α2b at several concentrations increased the proliferation inhibitory rate and total apoptosis rate of cells overexpressing SPOP. The findings of the present study showed that the SPOP protein was significantly expressed at low levels in most clear cell RCC (ccRCC) tissues and at relatively high levels in the majority of adjacent normal tissues and kidney tissues. Kaplan-Meier survival analysis showed that there was no statistically significant difference in cumulative survival based on the data of different SPOP expression levels in TMA and patients.ConclusionsIn contrast to previous studies, our findings demonstrated that overexpression of SPOP might suppress the progression of RCC cells, which was supported by cell experiments and immunohistochemical staining. SPOP could be a potential tumour inhibitor in RCC.

Dataset Information

The association of speckle-type POZ protein with lymph node metastasis and prognosis in cancer patients: A meta-analysis.

Publications

The association of speckle-type POZ protein with lymph node metastasis and prognosis in cancer patients: A meta-analysis.

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