Assessing fractional hair cell survival in archival human temporal bones.

ABSTRACT:

Objectives/hypothesis

Histopathological analysis of hair cell survival in human temporal bone sections has historically been binarized such that each hair cell row is rated as either present or absent, thereby greatly underestimating the amount of hair cell loss. Here, we describe and validate a technique to reliably assess fractional hair cell survival in archival sections stained with hematoxylin and eosin (H&E) using high-resolution light microscopy and optical sectioning.

Study design

Technique validation.

Methods

Hair cell counts in archival temporal bone slide sets were performed by several observers using either differential interference contrast (DIC) or confocal microscopy of the endogenous eosin fluorescence in hair cells. As a further cross-check, additiona

SUBMITTER: Wu PZ 

PROVIDER: S-EPMC6783317 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature