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Characterization of a Carbapenem-Resistant Kluyvera Cryocrescens Isolate Carrying Blandm-1 from Hospital Sewage.


ABSTRACT: Carbapenem-resistant Enterobacteriaceae have been a global public health issue in recent years. Here, a carbapenem-resistant Kluyvera cryocrescens strain SCW13 was isolated from hospital sewage, and was then subjected to whole-genome sequencing (WGS). Based on WGS data, antimicrobial resistance genes were identified. Resistance plasmids were completely circularized and further bioinformatics analyses of plasmids were performed. A conjugation assay was performed to identify a self-transmissible plasmid mediating carbapenem resistance. A phylogenetic tree was constructed based on the core genome of publicly available Kluyvera strains. The isolate SCW13 exhibited resistance to cephalosporin and carbapenem. blaNDM-1 was found to be located on a ~53-kb self-transmissible IncX3 plasmid, which exhibited high similarity to the previously reported pNDM-HN380, which is an epidemic blaNDM-1-carrying IncX3 plasmid. Further, we found that SCW13 contained a chromosomal blaKLUC-2 gene, which was the probable origin of the plasmid-born blaKLUC-2 found in Enterobacter cloacae. Phylogenetic analysis showed that K. cryocrescens SCW13 exhibited a close relationship with K. cryocrescens NCTC10483. These findings highlight the further dissemination of blaNDM through clonal IncX3 plasmids related to pNDM-HN380 among uncommon Enterobacteriaceae strains, including Kluyvera in this case.

SUBMITTER: Li Y 

PROVIDER: S-EPMC6783849 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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Characterization of a Carbapenem-Resistant <i>Kluyvera Cryocrescens</i> Isolate Carrying <i>Bla</i><sub>ndm-1</sub> from Hospital Sewage.

Li Ying Y   Luo Li L   Xiao Zhijiao Z   Wang Guangxi G   Li Chengwen C   Zhang Zhikun Z   Zhou Yingshun Y   Zhang Luhua L  

Antibiotics (Basel, Switzerland) 20190916 3


Carbapenem-resistant <i>Enterobacteriaceae</i> have been a global public health issue in recent years. Here, a carbapenem-resistant <i>Kluyvera cryocrescens</i> strain SCW13 was isolated from hospital sewage, and was then subjected to whole-genome sequencing (WGS). Based on WGS data, antimicrobial resistance genes were identified. Resistance plasmids were completely circularized and further bioinformatics analyses of plasmids were performed. A conjugation assay was performed to identify a self-t  ...[more]

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