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OMIP-044: 28-color immunophenotyping of the human dendritic cell compartment.

ABSTRACT: This work describes the first 30-parameter immunophenotyping of the human dendritic cell (DC) compartment using fluorescent-based flow cytometry. The optimized panel allows for simultaneous detection of 21 myeloid-centric markers distinguishing all canonical DC subsets, with parallel enumeration of monocytes, T and B cells as well as NK cells. Thus, this panel will be useful for extensive phenotyping of immune cells from a variety of human samples limited in size.

SUBMITTER: Mair F

PROVIDER: S-EPMC6785241 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature

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OMIP-044: 28-color immunophenotyping of the human dendritic cell compartment.

Mair Florian F Prlic Martin M

Cytometry. Part A : the journal of the International Society for Analytical Cytology 20180122 4

This work describes the first 30-parameter immunophenotyping of the human dendritic cell (DC) compartment using fluorescent-based flow cytometry. The optimized panel allows for simultaneous detection of 21 myeloid-centric markers distinguishing all canonical DC subsets, with parallel enumeration of monocytes, T and B cells as well as NK cells. Thus, this panel will be useful for extensive phenotyping of immune cells from a variety of human samples limited in size. ...[more]

PMID: 29356334

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