Single cell analysis of nutrient regulation of Clostridioides (Clostridium) difficile motility.
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ABSTRACT:
SUBMITTER: Courson DS
PROVIDER: S-EPMC6785396 | biostudies-literature | 2019 Oct
REPOSITORIES: biostudies-literature
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Single cell analysis of nutrient regulation of Clostridioides (Clostridium) difficile motility.
Courson David S DS Pokhrel Astha A Scott Cody C Madrill Melissa M Rinehold Alden J AJ Tamayo Rita R Cheney Richard E RE Purcell Erin B EB
Anaerobe 20190803
Regulation of bacterial motility to maximize nutrient acquisition or minimize exposure to harmful substances plays an important role in microbial proliferation and host colonization. The technical difficulties of performing high-resolution live microscopy on anaerobes have hindered mechanistic studies of motility in Clostridioides (formerly Clostridium) difficile. Here, we present a widely applicable protocol for live cell imaging of anaerobic bacteria that has allowed us to characterize C. diff ...[more]
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