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Repair of multiple simultaneous double-strand breaks causes bursts of genome-wide clustered hypermutation.


ABSTRACT: A single cancer genome can harbor thousands of clustered mutations. Mutation signature analyses have revealed that the origin of clusters are lesions in long tracts of single-stranded (ss) DNA damaged by apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like (APOBEC) cytidine deaminases, raising questions about molecular mechanisms that generate long ssDNA vulnerable to hypermutation. Here, we show that ssDNA intermediates formed during the repair of gamma-induced bursts of double-strand breaks (DSBs) in the presence of APOBEC3A in yeast lead to multiple APOBEC-induced clusters similar to cancer. We identified three independent pathways enabling cluster formation associated with repairing bursts of DSBs: 5' to 3' bidirectional resection, unidirectional resection, and break-induced replication (BIR). Analysis of millions of mutations in APOBEC-hypermutated cancer genomes revealed that cancer tolerance to formation of hypermutable ssDNA is similar to yeast and that the predominant pattern of clustered mutagenesis is the same as in resection-defective yeast, suggesting that cluster formation in cancers is driven by a BIR-like mechanism. The phenomenon of genome-wide burst of clustered mutagenesis revealed by our study can play an important role in generating somatic hypermutation in cancers as well as in noncancerous cells.

SUBMITTER: Sakofsky CJ 

PROVIDER: S-EPMC6786661 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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Repair of multiple simultaneous double-strand breaks causes bursts of genome-wide clustered hypermutation.

Sakofsky Cynthia J CJ   Saini Natalie N   Klimczak Leszek J LJ   Chan Kin K   Malc Ewa P EP   Mieczkowski Piotr A PA   Burkholder Adam B AB   Fargo David D   Gordenin Dmitry A DA  

PLoS biology 20190930 9


A single cancer genome can harbor thousands of clustered mutations. Mutation signature analyses have revealed that the origin of clusters are lesions in long tracts of single-stranded (ss) DNA damaged by apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like (APOBEC) cytidine deaminases, raising questions about molecular mechanisms that generate long ssDNA vulnerable to hypermutation. Here, we show that ssDNA intermediates formed during the repair of gamma-induced bursts of double-stra  ...[more]

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