Dataset Information

Overexpressing the Myrosinase Gene TGG1 Enhances Stomatal Defense Against Pseudomonas syringae and Delays Flowering in Arabidopsis.

ABSTRACT: Myrosinase enzymes and their substrate glucosinolates provide a specific defensive mechanism against biotic invaders in the Brassicaceae family. In these plants, myrosinase hydrolyzes glucosinolates into diverse products, which can have direct antibiotic activity or function as signaling molecules that initiate a variety of defense reactions. A myrosinase, ?-thioglucoside glucohydrolase 1 (TGG1) was previously found to be strikingly abundant in guard cells, and it is required for the abscisic acid (ABA) response of stomata. However, it remains unknown which particular physiological processes actually involve stomatal activity as modulated by TGG1. In this experimental study, a homologous TGG1 gene from broccoli (Brassica oleracea var. italica), BoTGG1, was overexpressed in Arabidopsis. The transgenic plants showed enhanced resistance against the bacterial pathogen Pseudomonas syringae pv. tomato (Pst) DC3000 via improved stomatal defense. Upon Pst DC3000 infection, overexpressing BoTGG1 accelerated stomatal closure and inhibited the reopening of stomata. Compared with the wild type, 35S::BoTGG1 was more sensitive to ABA- and salicylic acid (SA)-induced stomatal closure but was less sensitive to indole-3-acetic acid (IAA)-inhibited stomatal closure, thus indicating these hormone signaling pathways were possibly involved in stomatal defense regulated by TGG1. Furthermore, overexpression of BoTGG1 delayed flowering by promoting the expression of FLOWERING LOCUS C (FLC), which encodes a MADS-box transcription factor known as floral repressor. Taken together, our study's results suggest glucosinolate metabolism mediated by TGG1 plays a role in plant stomatal defense against P. syringae and also modulates flowering time by affecting the FLC pathway.

SUBMITTER: Zhang K

PROVIDER: S-EPMC6787276 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Overexpressing the Myrosinase Gene <i>TGG1</i> Enhances Stomatal Defense Against <i>Pseudomonas syringae</i> and Delays Flowering in Arabidopsis.

Zhang Kaixin K Su Hongzhu H Zhou Jianxin J Liang Wenjie W Liu Desheng D Li Jing J

Frontiers in plant science 20191004

Myrosinase enzymes and their substrate glucosinolates provide a specific defensive mechanism against biotic invaders in the Brassicaceae family. In these plants, myrosinase hydrolyzes glucosinolates into diverse products, which can have direct antibiotic activity or function as signaling molecules that initiate a variety of defense reactions. A myrosinase, β-thioglucoside glucohydrolase 1 (TGG1) was previously found to be strikingly abundant in guard cells, and it is required for the abscisic ac ...[more]

PMID: 31636648

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Project description:Plant-pathogen interactions involve sophisticated action and counteraction strategies from both parties. Plants can recognize pathogen derived molecules, such as conserved pathogen associated molecular patterns (PAMPs) and effector proteins, and subsequently activate PAMP-triggered immunity (PTI) and effector-triggered immunity (ETI), respectively. However, pathogens can evade such recognitions and suppress host immunity with effectors, causing effector-triggered susceptibility (ETS). The differences among PTI, ETS, and ETI have not been completely understood. Toward a better understanding of PTI, ETS, and ETI, we systematically examined various defense-related phenotypes of Arabidopsis infected with different Pseudomonas syringae pv. maculicola ES4326 strains, using the virulence strain DG3 to induce ETS, the avirulence strain DG34 that expresses avrRpm1 (recognized by the resistance protein RPM1) to induce ETI, and HrcC(-) that lacks the type three secretion system to activate PTI. We found that plants infected with different strains displayed dynamic differences in the accumulation of the defense signaling molecule salicylic acid, expression of the defense marker gene PR1, cell death formation, and accumulation/localization of the reactive oxygen species, H2O2. The differences between PTI, ETS, and ETI are dependent on the doses of the strains used. These data support the quantitative nature of PTI, ETS, and ETI and they also reveal qualitative differences between PTI, ETS, and ETI. Interestingly, we observed the induction of large cells in the infected leaves, most obviously with HrcC(-) at later infection stages. The enlarged cells have increased DNA content, suggesting a possible activation of endoreplication. Consistent with strong induction of abnormal cell growth by HrcC(-), we found that the PTI elicitor flg22 also activates abnormal cell growth, depending on a functional flg22-receptor FLS2. Thus, our study has revealed a comprehensive picture of dynamic changes of defense phenotypes and cell fate determination during Arabidopsis-P. syringae interactions, contributing to a better understanding of plant defense mechanisms.

Project description:Bacterial infection of plants often begins with colonization of the plant surface, followed by entry into the plant through wounds and natural openings (such as stomata), multiplication in the intercellular space (apoplast) of the infected tissues, and dissemination of bacteria to other plants. Historically, most studies assess bacterial infection based on final outcomes of disease and/or pathogen growth using whole infected tissues; few studies have genetically distinguished the contribution of different host cell types in response to an infection. The phytotoxin coronatine (COR) is produced by several pathovars of Pseudomonas syringae. COR-deficient mutants of P. s. tomato (Pst) DC3000 are severely compromised in virulence, especially when inoculated onto the plant surface. We report here a genetic screen to identify Arabidopsis mutants that could rescue the virulence of COR-deficient mutant bacteria. Among the susceptible to coronatine-deficient Pst DC3000 (scord) mutants were two that were defective in stomatal closure response, two that were defective in apoplast defense, and four that were defective in both stomatal and apoplast defense. Isolation of these three classes of mutants suggests that stomatal and apoplastic defenses are integrated in plants, but are genetically separable, and that COR is important for Pst DC3000 to overcome both stomatal guard cell- and apoplastic mesophyll cell-based defenses. Of the six mutants defective in bacterium-triggered stomatal closure, three are defective in salicylic acid (SA)-induced stomatal closure, but exhibit normal stomatal closure in response to abscisic acid (ABA), and scord7 is compromised in both SA- and ABA-induced stomatal closure. We have cloned SCORD3, which is required for salicylic acid (SA) biosynthesis, and SCORD5, which encodes an ATP-binding cassette (ABC) protein, AtGCN20/AtABCF3, predicted to be involved in stress-associated protein translation control. Identification of SCORD5 begins to implicate an important role of stress-associated protein translation in stomatal guard cell signaling in response to microbe-associated molecular patterns and bacterial infection.

Dataset Information

Overexpressing the Myrosinase Gene TGG1 Enhances Stomatal Defense Against Pseudomonas syringae and Delays Flowering in Arabidopsis.

Publications

Overexpressing the Myrosinase Gene <i>TGG1</i> Enhances Stomatal Defense Against <i>Pseudomonas syringae</i> and Delays Flowering in Arabidopsis.

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