A New Escherichia coli Entry Vector Series (pIIS18) for Seamless Gene Cloning Using Type IIS Restriction Enzymes.
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ABSTRACT:
SUBMITTER: Altaib H
PROVIDER: S-EPMC6787310 | biostudies-literature | 2019 Oct
REPOSITORIES: biostudies-literature
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A New Escherichia coli Entry Vector Series (pIIS18) for Seamless Gene Cloning Using Type IIS Restriction Enzymes.
Altaib Hend H Ozaki Yuka Y Kozakai Tomoya T Badr Yassien Y Nomura Izumi I Suzuki Tohru T
Microbiology resource announcements 20191010 41
A series of new <i>Escherichia coli</i> entry vectors (pIIS18-SapI, pIIS18-BsmBI, pIIS18-BsaI, pIIS18-BfuAI-1, and pIIS18-BfuAI-2) was constructed based on a modified pUC18 backbone, which carried newly designed multiple cloning sites, consisting of two facing type IIS enzyme cleavage sites and one blunt-end enzyme cleavage site. These vectors are useful for seamless gene cloning. ...[more]
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