Dataset Information

Integrins Have Cell-Type-Specific Roles in the Development of Motor Neuron Connectivity.

ABSTRACT: Formation of the nervous system requires a complex series of events including proper extension and guidance of neuronal axons and dendrites. Here we investigate the requirement for integrins, a class of transmembrane cell adhesion receptors, in regulating these processes across classes of C. elegans motor neurons. We show ? integrin/ina-1 is expressed by both GABAergic and cholinergic motor neurons. Despite this, our analysis of hypomorphic ina-1(gm144) mutants indicates preferential involvement of ? integrin/ina-1 in GABAergic commissural development, without obvious involvement in cholinergic commissural development. The defects in GABAergic commissures of ina-1(gm144) mutants included both premature termination and guidance errors and were reversed by expression of wild type ina-1 under control of the native ina-1 promoter. Our results also show that ? integrin/ina-1 is important for proper outgrowth and guidance of commissures from both embryonic and post-embryonic born GABAergic motor neurons, indicating an ongoing requirement for integrin through two phases of GABAergic neuron development. Our findings provide insights into neuron-specific roles for integrin that would not be predicted based solely upon expression analysis.

SUBMITTER: Oliver D

PROVIDER: S-EPMC6787651 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature

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Integrins Have Cell-Type-Specific Roles in the Development of Motor Neuron Connectivity.

Oliver Devyn D Norman Emily E Bates Heather H Avard Rachel R Rettler Monika M Bénard Claire Y CY Francis Michael M MM Lemons Michele L ML

Journal of developmental biology 20190827 3

Formation of the nervous system requires a complex series of events including proper extension and guidance of neuronal axons and dendrites. Here we investigate the requirement for integrins, a class of transmembrane cell adhesion receptors, in regulating these processes across classes of C. elegans motor neurons. We show α integrin/ina-1 is expressed by both GABAergic and cholinergic motor neurons. Despite this, our analysis of hypomorphic ina-1(gm144) mutants indicates pre ...[more]

PMID: 31461926

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Dataset Information

Integrins Have Cell-Type-Specific Roles in the Development of Motor Neuron Connectivity.

Publications

Integrins Have Cell-Type-Specific Roles in the Development of Motor Neuron Connectivity.

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