Ontology highlight
ABSTRACT:
SUBMITTER: Whittle EE
PROVIDER: S-EPMC6787898 | biostudies-literature | 2019
REPOSITORIES: biostudies-literature
Whittle Emily E EE Legood Simon W SW Alav Ilyas I Dulyayangkul Punyawee P Overton Tim W TW Blair Jessica M A JMA
Frontiers in microbiology 20191004
Gram-negative infections are increasingly difficult to treat because of their impermeable outer membranes (OM) and efflux pumps which maintain a low intracellular accumulation of antibiotics within cells. Historically, measurement of accumulation of drugs or dyes within Gram-negative cells has concentrated on analyzing whole bacterial populations. Here, we have developed a method to measure the intracellular accumulation of ethidium bromide, a fluorescent DNA intercalating dye, in single cells u ...[more]