Dataset Information

Rapid and Noninvasive Typing and Assessment of Nutrient Content of Maize Kernels Using a Handheld Raman Spectrometer.

ABSTRACT: To thrive as a global civilization, food production must meet the demands of our ever-growing population. There are more than a billion people on the planet suffering from malnutrition through poor quality or lack of food. Nutrient content of food can be determined by a variety of methods, which have issues such as slow analysis or sample destruction. Near-infrared (NIR) spectroscopy is a long-standing alternative to these methods. In this work, we demonstrated that Raman spectroscopy (RS), another spectroscopic method, can also be used to assess the nutrient content of maize (Zea mays), one of the most widely cultivated grains in the world. Using a handheld Raman spectrometer, we predicted the content of carbohydrates, fibers, carotenoids, and proteins in six different varieties of maize. This analysis requires only a single maize kernel and is fast (1s), portable, noninvasive, and nondestructive. Moreover, we showed that RS in combination with chemometric methods can be used for highly accurate (approximately 90%) spectroscopic typing of maize, which is important for plant breeders and farmers. Finally, we demonstrate that Raman-based approach is as accurate as NIR analysis. These findings suggest that portable Raman systems can be used on combines and grain elevators for autonomous control of grain quality.

SUBMITTER: Krimmer M

PROVIDER: S-EPMC6787905 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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Rapid and Noninvasive Typing and Assessment of Nutrient Content of Maize Kernels Using a Handheld Raman Spectrometer.

Krimmer Mark M Farber Charles C Kurouski Dmitry D

ACS omega 20190926 15

To thrive as a global civilization, food production must meet the demands of our ever-growing population. There are more than a billion people on the planet suffering from malnutrition through poor quality or lack of food. Nutrient content of food can be determined by a variety of methods, which have issues such as slow analysis or sample destruction. Near-infrared (NIR) spectroscopy is a long-standing alternative to these methods. In this work, we demonstrated that Raman spectroscopy (RS), anot ...[more]

PMID: 31616810

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Project description:BACKGROUND: Kernel moisture at harvest is an important trait since a low value is required to prevent unexpected early germination and ensure seed preservation. It is also well known that early germination occurs in viviparous mutants, which are impaired in abscisic acid (ABA) biosynthesis. To provide some insight into the genetic determinism of kernel desiccation in maize, quantitative trait loci (QTLs) were detected for traits related to kernel moisture and ABA content in both embryo and endosperm during kernel desiccation. In parallel, the expression and mapping of genes involved in kernel desiccation and ABA biosynthesis, were examined to detect candidate genes. RESULTS: The use of an intermated recombinant inbred line population allowed for precise QTL mapping. For 29 traits examined in an unreplicated time course trial of days after pollination, a total of 78 QTLs were detected, 43 being related to kernel desiccation, 15 to kernel weight and 20 to ABA content. Multi QTL models explained 35 to 50% of the phenotypic variation for traits related to water status, indicating a large genetic control amenable to breeding. Ten of the 20 loci controlling ABA content colocated with previously detected QTLs controlling water status and ABA content in water stressed leaves. Mapping of candidate genes associated with kernel desiccation and ABA biosynthesis revealed several colocations between genes with putative functions and QTLs. Parallel investigation via RT-PCR experiments showed that the expression patterns of the ABA-responsive Rab17 and Rab28 genes as well as the late embryogenesis abundant Emb5 and aquaporin genes were related to desiccation rate and parental allele effect. Database searches led to the identification and mapping of two zeaxanthin epoxidase (ZEP) and five novel 9-cis-epoxycarotenoid dioxygenase (NCED) related genes, both gene families being involved in ABA biosynthesis. The expression of these genes appeared independent in the embryo and endosperm and not correlated with ABA content in either tissue. CONCLUSIONS: A high resolution QTL map for kernel desiccation and ABA content in embryo and endosperm showed several precise colocations between desiccation and ABA traits. Five new members of the maize NCED gene family and another maize ZEP gene were identified and mapped. Among all the identified candidates, aquaporins and members of the Responsive to ABA gene family appeared better candidates than NCEDs and ZEPs.

Dataset Information

Rapid and Noninvasive Typing and Assessment of Nutrient Content of Maize Kernels Using a Handheld Raman Spectrometer.

Publications

Rapid and Noninvasive Typing and Assessment of Nutrient Content of Maize Kernels Using a Handheld Raman Spectrometer.

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