Amurensin H, a Derivative From Resveratrol, Ameliorates Lipopolysaccharide/Cigarette Smoke-Induced Airway Inflammation by Blocking the Syk/NF-?B Pathway.

ABSTRACT: Amurensin H, a resveratrol dimer derived from Vitis amurensis Rupr, has several biological effects, including anti-inflammatory and antioxidant activities. Studies have found that amurensin H attenuated asthma-like allergic airway inflammation. However, its protective activity on chronic obstructive pulmonary disease (COPD) airway inflammation is not fully explored. The present study used a lipopolysaccharide (LPS)/cigarette smoke-induced mice model and an LPS-stimulated THP-1-derived macrophages model to measure the lung tissue's morphology changes. The results showed that amurensin H ameliorated the histological inflammatory alterations in the lung tissues, leading to a decrease in the expression of interleukin 6 (IL-6), IL-17A, tumor necrosis factor ? (TNF-?), and interferon ? in bronchoalveolar lavage fluid. Amurensin H also significantly inhibited the release of IL-1?, IL-6, IL-8, and TNF-? in LPS-stimulated THP-1-derived macrophages. Furthermore, amurensin H markedly inhibited the expressions of p-Syk, nuclear factor ?B (NF-?B), and p-NF-?B both in vivo and in vitro. Results from cotreatment with Syk inhibitor BAY61-3606 and NF-?B inhibitor BAY11-7082 in vitro revealed that amurensin H's protective effect against airway inflammation could be due partly to the inhibition of the Syk/NF-?B pathway. These findings suggest that amurensin H shows therapeutic effects on COPD airway inflammation, and inhibiting the Syk/NF-?B pathway might be part of its underlying mechanisms.

SUBMITTER: Fan Y 

PROVIDER: S-EPMC6787933 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature