Dataset Information

A functional regulatory variant of MYH3 influences muscle fiber-type composition and intramuscular fat content in pigs.

ABSTRACT: Muscle development and lipid accumulation in muscle critically affect meat quality of livestock. However, the genetic factors underlying myofiber-type specification and intramuscular fat (IMF) accumulation remain to be elucidated. Using two independent intercrosses between Western commercial breeds and Korean native pigs (KNPs) and a joint linkage-linkage disequilibrium analysis, we identified a 488.1-kb region on porcine chromosome 12 that affects both reddish meat color (a*) and IMF. In this critical region, only the MYH3 gene, encoding myosin heavy chain 3, was found to be preferentially overexpressed in the skeletal muscle of KNPs. Subsequently, MYH3-transgenic mice demonstrated that this gene controls both myofiber-type specification and adipogenesis in skeletal muscle. We discovered a structural variant in the promotor/regulatory region of MYH3 for which Q allele carriers exhibited significantly higher values of a* and IMF than q allele carriers. Furthermore, chromatin immunoprecipitation and cotransfection assays showed that the structural variant in the 5'-flanking region of MYH3 abrogated the binding of the myogenic regulatory factors (MYF5, MYOD, MYOG, and MRF4). The allele distribution of MYH3 among pig populations worldwide indicated that the MYH3 Q allele is of Asian origin and likely predates domestication. In conclusion, we identified a functional regulatory sequence variant in porcine MYH3 that provides novel insights into the genetic basis of the regulation of myofiber type ratios and associated changes in IMF in pigs. The MYH3 variant can play an important role in improving pork quality in current breeding programs.

SUBMITTER: Cho IC

PROVIDER: S-EPMC6788688 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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A functional regulatory variant of MYH3 influences muscle fiber-type composition and intramuscular fat content in pigs.

Cho In-Cheol IC Park Hee-Bok HB Ahn Jin Seop JS Han Sang-Hyun SH Lee Jae-Bong JB Lim Hyun-Tae HT Yoo Chae-Kyoung CK Jung Eun-Ji EJ Kim Dong-Hwan DH Sun Wu-Sheng WS Ramayo-Caldas Yuliaxis Y Kim Sang-Geum SG Kang Yong-Jun YJ Kim Yoo-Kyung YK Shin Hyun-Sook HS Seong Pil-Nam PN Hwang In-Sul IS Park Beom-Young BY Hwang Seongsoo S Lee Sung-Soo SS Ryu Youn-Chul YC Lee Jun-Heon JH Ko Moon-Suck MS Lee Kichoon K Andersson Göran G Pérez-Enciso Miguel M Lee Jeong-Woong JW

PLoS genetics 20191011 10

Muscle development and lipid accumulation in muscle critically affect meat quality of livestock. However, the genetic factors underlying myofiber-type specification and intramuscular fat (IMF) accumulation remain to be elucidated. Using two independent intercrosses between Western commercial breeds and Korean native pigs (KNPs) and a joint linkage-linkage disequilibrium analysis, we identified a 488.1-kb region on porcine chromosome 12 that affects both reddish meat color (a*) and IMF. In this c ...[more]

PMID: 31603892

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Dataset Information

A functional regulatory variant of MYH3 influences muscle fiber-type composition and intramuscular fat content in pigs.

Publications

A functional regulatory variant of MYH3 influences muscle fiber-type composition and intramuscular fat content in pigs.

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