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Proteomic analysis of Escherichia coli detergent-resistant membranes (DRM).

ABSTRACT: Membrane microdomains or lipid rafts compartmentalize cellular processes by laterally organizing membrane components. Such sub-membrane structures were mainly described in eukaryotic cells, but, recently, also in bacteria. Here, the protein content of lipid rafts in Escherichia coli was explored by mass spectrometry analyses of Detergent Resistant Membranes (DRM). We report that at least three of the four E. coli flotillin homologous proteins were found to reside in DRM, along with 77 more proteins. Moreover, the proteomic data were validated by subcellular localization, using immunoblot assays and fluorescence microscopy of selected proteins. Our results confirm the existence of lipid raft-like microdomains in the inner membrane of E. coli and represent the first comprehensive profiling of proteins in these bacterial membrane platforms.

SUBMITTER: Guzman-Flores JE

PROVIDER: S-EPMC6788730 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Proteomic analysis of Escherichia coli detergent-resistant membranes (DRM).

Guzmán-Flores José E JE Steinemann-Hernández Lidia L González de la Vara Luis E LE Gavilanes-Ruiz Marina M Romeo Tony T Alvarez Adrián F AF Georgellis Dimitris D

PloS one 20191011 10

Membrane microdomains or lipid rafts compartmentalize cellular processes by laterally organizing membrane components. Such sub-membrane structures were mainly described in eukaryotic cells, but, recently, also in bacteria. Here, the protein content of lipid rafts in Escherichia coli was explored by mass spectrometry analyses of Detergent Resistant Membranes (DRM). We report that at least three of the four E. coli flotillin homologous proteins were found to reside in DRM, along with 77 more prote ...[more]

PMID: 31603938

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Proteomic Analysis of Lipid Raft-Like Detergent-Resistant Membranes of Lens Fiber Cells.

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