Ontology highlight
ABSTRACT:
SUBMITTER: Angarola B
PROVIDER: S-EPMC6789162 | biostudies-literature | 2019 Oct
REPOSITORIES: biostudies-literature
Angarola Brittany B Ferguson Shawn M SM
Molecular biology of the cell 20190918 22
Stable localization of the Rheb GTPase to lysosomes is thought to be required for activation of mTOR complex 1 (mTORC1) signaling. However, the lysosome targeting mechanisms for Rheb remain unclear. We therefore investigated the relationship between Rheb subcellular localization and mTORC1 activation. Surprisingly, we found that Rheb was undetectable at lysosomes. Nonetheless, functional assays in knockout human cells revealed that farnesylation of the C-terminal CaaX motif on Rheb was essential ...[more]