Ontology highlight
ABSTRACT:
SUBMITTER: Riley LA
PROVIDER: S-EPMC6791906 | biostudies-literature | 2019 Oct
REPOSITORIES: biostudies-literature
Riley Lauren A LA Ji Lexiang L Schmitz Robert J RJ Westpheling Janet J Guss Adam M AM
Journal of industrial microbiology & biotechnology 20190724 9-10
A major barrier to both metabolic engineering and fundamental biological studies is the lack of genetic tools in most microorganisms. One example is Clostridium thermocellum ATCC 27405<sup>T</sup>, where genetic tools are not available to help validate decades of hypotheses. A significant barrier to DNA transformation is restriction-modification systems, which defend against foreign DNA methylated differently than the host. To determine the active restriction-modification systems in this strain, ...[more]