Ontology highlight
ABSTRACT: Background
Prenatal exposure to air pollution has been associated with childhood respiratory disease and other adverse outcomes. Epigenetics is a suggested link between exposures and health outcomes.Objectives
We aimed to investigate associations between prenatal exposure to particulate matter (PM) with diameter [Formula: see text] ([Formula: see text]) or [Formula: see text] ([Formula: see text]) and DNA methylation in newborns and children.Methods
We meta-analyzed associations between exposure to [Formula: see text] ([Formula: see text]) and [Formula: see text] ([Formula: see text]) at maternal home addresses during pregnancy and newborn DNA methylation assessed by Illumina Infinium HumanMethylation450K BeadChip in nine European and American studies, with replication in 688 independent newborns and look-up analyses in 2,118 older children. We used two approaches, one focusing on single cytosine-phosphate-guanine (CpG) sites and another on differentially methylated regions (DMRs). We also related PM exposures to blood mRNA expression.Results
Six CpGs were significantly associated [false discovery rate (FDR) [Formula: see text]] with prenatal [Formula: see text] and 14 with [Formula: see text] exposure. Two of the [Formula: see text] CpGs mapped to FAM13A (cg00905156) and NOTCH4 (cg06849931) previously associated with lung function and asthma. Although these associations did not replicate in the smaller newborn sample, both CpGs were significant ([Formula: see text]) in 7- to 9-y-olds. For cg06849931, however, the direction of the association was inconsistent. Concurrent [Formula: see text] exposure was associated with a significantly higher NOTCH4 expression at age 16 y. We also identified several DMRs associated with either prenatal [Formula: see text] and or [Formula: see text] exposure, of which two [Formula: see text] DMRs, including H19 and MARCH11, replicated in newborns.Conclusions
Several differentially methylated CpGs and DMRs associated with prenatal PM exposure were identified in newborns, with annotation to genes previously implicated in lung-related outcomes. https://doi.org/10.1289/EHP4522.
SUBMITTER: Gruzieva O
PROVIDER: S-EPMC6792178 | biostudies-literature | 2019 May
REPOSITORIES: biostudies-literature
Gruzieva Olena O Xu Cheng-Jian CJ Yousefi Paul P Relton Caroline C Merid Simon Kebede SK Breton Carrie V CV Gao Lu L Volk Heather E HE Feinberg Jason I JI Ladd-Acosta Christine C Bakulski Kelly K Auffray Charles C Lemonnier Nathanaël N Plusquin Michelle M Ghantous Akram A Herceg Zdenko Z Nawrot Tim S TS Pizzi Costanza C Richiardi Lorenzo L Rusconi Franca F Vineis Paolo P Kogevinas Manolis M Felix Janine F JF Duijts Liesbeth L den Dekker Herman T HT Jaddoe Vincent W V VWV Ruiz José L JL Bustamante Mariona M Antó Josep Maria JM Sunyer Jordi J Vrijheid Martine M Gutzkow Kristine B KB Grazuleviciene Regina R Hernandez-Ferrer Carles C Annesi-Maesano Isabella I Lepeule Johanna J Bousquet Jean J Bergström Anna A Kull Inger I Söderhäll Cilla C Kere Juha J Gehring Ulrike U Brunekreef Bert B Just Allan C AC Wright Rosalind J RJ Peng Cheng C Gold Diane R DR Kloog Itai I DeMeo Dawn L DL Pershagen Göran G Koppelman Gerard H GH London Stephanie J SJ Baccarelli Andrea A AA Melén Erik E
Environmental health perspectives 20190531 5
<h4>Background</h4>Prenatal exposure to air pollution has been associated with childhood respiratory disease and other adverse outcomes. Epigenetics is a suggested link between exposures and health outcomes.<h4>Objectives</h4>We aimed to investigate associations between prenatal exposure to particulate matter (PM) with diameter [Formula: see text] ([Formula: see text]) or [Formula: see text] ([Formula: see text]) and DNA methylation in newborns and children.<h4>Methods</h4>We meta-analyzed assoc ...[more]