Dataset Information

The HD-Zip transcription factor LcHB2 regulates litchi fruit abscission through the activation of two cellulase genes.

ABSTRACT: Cellulases play important roles in the shedding of plant organs; however, little is yet known about the functions of cellulase genes during the process of organ abscission. Abnormal fruitlet abscission is a serious problem in the production of litchi (Litchi chinensis), an economically important fruit widely grown in South Asia. In this study, two abscission-accelerating treatments (carbohydrate stress and application of ethephon) were evaluated in litchi fruitlets. Cell wall degradation and cell separation were clearly observed in the abscission zones of treated fruitlets, consistent with enhanced cellulase activities and reduced cellulose contents. The expression of two cellulase genes (LcCEL2 and LcCEL8) was strongly associated with abscission. Floral organs of transgenic Arabidopsis overexpressing LcCEL2 or LcCEL8 showed remarkably precocious abscission. Electrophoretic mobility shift assays and transient expression experiments demonstrated that a novel homeodomain-leucine zipper transcription factor, LcHB2, could directly bind to and activate HD-binding cis-elements in the LcCEL2 and LcCEL8 promoters. Our results provide new information regarding the transcriptional regulation of the cellulase genes responsible for cell wall degradation and cell separation during plant organ shedding, and raise the possibility of future manipulation of litchi fruitlet abscission by modulation of the activities of these two cellulases.

SUBMITTER: Li C

PROVIDER: S-EPMC6793447 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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The HD-Zip transcription factor LcHB2 regulates litchi fruit abscission through the activation of two cellulase genes.

Li Caiqin C Zhao Minglei M Ma Xingshuai X Wen Zhenxi Z Ying Peiyuan P Peng Manjun M Ning Xiping X Xia Rui R Wu Hong H Li Jianguo J

Journal of experimental botany 20191001 19

Cellulases play important roles in the shedding of plant organs; however, little is yet known about the functions of cellulase genes during the process of organ abscission. Abnormal fruitlet abscission is a serious problem in the production of litchi (Litchi chinensis), an economically important fruit widely grown in South Asia. In this study, two abscission-accelerating treatments (carbohydrate stress and application of ethephon) were evaluated in litchi fruitlets. Cell wall degradation and cel ...[more]

PMID: 31173099

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Project description:BACKGROUND: Plant HD-Zip transcription factors are modular proteins in which a homeodomain is associated to a leucine zipper. Of the four subfamilies in which they are divided, the tested members from subfamily I bind in vitro the same pseudopalindromic sequence CAAT(A/T)ATTG and among them, several exhibit similar expression patterns. However, most experiments in which HD-Zip I proteins were over or ectopically expressed under the control of the constitutive promoter 35S CaMV resulted in transgenic plants with clearly different phenotypes. Aiming to elucidate the structural mechanisms underlying such observation and taking advantage of the increasing information in databases of sequences from diverse plant species, an in silico analysis was performed. In addition, some of the results were also experimentally supported. RESULTS: A phylogenetic tree of 178 HD-Zip I proteins together with the sequence conservation presented outside the HD-Zip domains allowed the distinction of six groups of proteins. A motif-discovery approach enabled the recognition of an activation domain in the carboxy-terminal regions (CTRs) and some putative regulatory mechanisms acting in the amino-terminal regions (NTRs) and CTRs involving sumoylation and phosphorylation. A yeast one-hybrid experiment demonstrated that the activation activity of ATHB1, a member of one of the groups, is located in its CTR. Chimerical constructs were performed combining the HD-Zip domain of one member with the CTR of another and transgenic plants were obtained with these constructs. The phenotype of the chimerical transgenic plants was similar to the observed in transgenic plants bearing the CTR of the donor protein, revealing the importance of this module inside the whole protein. CONCLUSIONS: The bioinformatical results and the experiments conducted in yeast and transgenic plants strongly suggest that the previously poorly analyzed NTRs and CTRs of HD-Zip I proteins play an important role in their function, hence potentially constituting a major source of functional diversity among members of this subfamily.

Dataset Information

The HD-Zip transcription factor LcHB2 regulates litchi fruit abscission through the activation of two cellulase genes.

Publications

The HD-Zip transcription factor LcHB2 regulates litchi fruit abscission through the activation of two cellulase genes.

Similar Datasets

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