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A bacterial pioneer produces cellulase complexes that persist through community succession.

ABSTRACT: Cultivation of microbial consortia provides low-complexity communities that can serve as tractable models to understand community dynamics. Time-resolved metagenomics demonstrated that an aerobic cellulolytic consortium cultivated from compost exhibited community dynamics consistent with the definition of an endogenous heterotrophic succession. The genome of the proposed pioneer population, 'Candidatus Reconcilibacillus cellulovorans', possessed a gene cluster containing multidomain glycoside hydrolases (GHs). Purification of the soluble cellulase activity from a 300litre cultivation of this consortium revealed that ~70% of the activity arose from the 'Ca. Reconcilibacillus cellulovorans' multidomain GHs assembled into cellulase complexes through glycosylation. These remarkably stable complexes have supramolecular structures for enzymatic cellulose hydrolysis that are distinct from cellulosomes. The persistence of these complexes during cultivation indicates that they may be active through multiple cultivations of this consortium and act as public goods that sustain the community. The provision of extracellular GHs as public goods may influence microbial community dynamics in native biomass-deconstructing communities relevant to agriculture, human health and biotechnology.

SUBMITTER: Kolinko S

PROVIDER: S-EPMC6794216 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature

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A bacterial pioneer produces cellulase complexes that persist through community succession.

Kolinko Sebastian S Wu Yu-Wei YW Tachea Firehiwot F Denzel Evelyn E Hiras Jennifer J Gabriel Raphael R Bäcker Nora N Chan Leanne Jade G LJG Eichorst Stephanie A SA Frey Dario D Chen Qiushi Q Azadi Parastoo P Adams Paul D PD Pray Todd R TR Tanjore Deepti D Petzold Christopher J CJ Gladden John M JM Simmons Blake A BA Singer Steven W SW

Nature microbiology 20171106 1

Cultivation of microbial consortia provides low-complexity communities that can serve as tractable models to understand community dynamics. Time-resolved metagenomics demonstrated that an aerobic cellulolytic consortium cultivated from compost exhibited community dynamics consistent with the definition of an endogenous heterotrophic succession. The genome of the proposed pioneer population, 'Candidatus Reconcilibacillus cellulovorans', possessed a gene cluster containing multidomain glycoside hy ...[more]

PMID: 29109478

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A bacterial pioneer produces cellulase complexes that persist through community succession.

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A bacterial pioneer produces cellulase complexes that persist through community succession.

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