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Long non-coding RNA HCG11 modulates glioma progression through cooperating with miR-496/CPEB3 axis.


ABSTRACT:

Objectives

It has been widely reported that long non-coding RNAs (lncRNAs) can participate in multiple biological processes of human cancers. lncRNA HLA complex group 11 (HCG11) has been reported in human cancers as a tumour suppressor. This study focused on investigating the function and mechanism of HCG11 in glioma.

Materials and methods

Based on The Cancer Genome Atlas (TCGA) data set and qRT-PCR analysis, the expression pattern of HCG11 was identified in glioma samples. The mechanism associated with HCG11 downregulation was determined by mechanism experiments. Gain-of-function assays were conducted for the identification of HCG11 function in glioma progression. Mechanism investigation based on the luciferase reporter assay, RIP assay and pull-down assay was used to explore the downstream molecular mechanism of HCG11. The role of molecular pathway in the progression of glioma was analysed in accordance with the rescue assays.

Results

HCG11 was expressed at low level in glioma samples compared with normal samples. FOXP1 could bind with HCG11 and transcriptionally inactivated HCG11. Overexpression of HCG11 efficiently suppressed cell proliferation, induced cell cycle arrest and promoted cell apoptosis. HCG11 was predominantly enriched in the cytoplasm of glioma cells and acted as a competing endogenous RNAs (ceRNAs) by sponging micro-496 to upregulate cytoplasmic polyadenylation element binding protein 3 (CPEB3). CEPB3 and miR-496 involved in HCG11-mediated glioma progression.

Conclusions

HCG11 inhibited glioma progression by regulating miR-496/CPEB3 axis.

SUBMITTER: Chen Y 

PROVIDER: S-EPMC6797506 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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Publications

Long non-coding RNA HCG11 modulates glioma progression through cooperating with miR-496/CPEB3 axis.

Chen Yangzong Y   Bao Chunchun C   Zhang Xiuxing X   Lin Xinshi X   Huang Hongou H   Wang Zhiqiang Z  

Cell proliferation 20190716 5


<h4>Objectives</h4>It has been widely reported that long non-coding RNAs (lncRNAs) can participate in multiple biological processes of human cancers. lncRNA HLA complex group 11 (HCG11) has been reported in human cancers as a tumour suppressor. This study focused on investigating the function and mechanism of HCG11 in glioma.<h4>Materials and methods</h4>Based on The Cancer Genome Atlas (TCGA) data set and qRT-PCR analysis, the expression pattern of HCG11 was identified in glioma samples. The me  ...[more]

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