Dataset Information

Differences in gene expression between high-grade dysplasia and invasive HPV⁺ and HPV^- tonsillar and base of tongue cancer.

ABSTRACT:

Background

Human papillomavirus (HPV) is a causative agent for tonsillar and base of tongue squamous cell carcinoma (TSCC/BOTSCC), as well as for cervical cancer. Premalignant stages in cervical cancer have been studied extensively, while little is known about premalignant stages in TSCC/BOTSCC and the role of HPV. Here we analyzed differences in gene and protein expression between high-grade dysplasia and invasive cancer in both HPV-positive (HPV⁺ ) and HPV-negative (HPV^- ) TSCC/BOTSCC.

Methods

High-grade dysplasia and invasive carcinoma were laser microdissected from HPV⁺ and HPV^- TSCC/BOTSCC tumor sections. Differential gene expression was studied utilizing nanoString RNA-panels and genes of interest were validated on the protein level by immunohistochemistry.

Results

Forty genes in the HPV⁺ tumors showed significantly different expression between high-grade dysplasia and invasive cancer and 33 genes in the HPV^- tumors. Five out of the nine most significant pathways showed similar increased activity in invasive cancer as compared to high-grade dysplasia in both HPV⁺ and HPV^- tumors. Lastly, significant differences in protein expression was confirmed for SPARC, psoriasin, type I collagen and galectin-1 in both HPV⁺ and HPV^- tumors.

Conclusions

This is to our knowledge the first study disclosing differences and similarities in gene expression between dysplastic and invasive HPV⁺ and HPV^- TSCC/BOTSCC.

SUBMITTER: Haeggblom L

PROVIDER: S-EPMC6797576 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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Publications

Differences in gene expression between high-grade dysplasia and invasive HPV<sup>+</sup> and HPV<sup>-</sup> tonsillar and base of tongue cancer.

Haeggblom Linnea L Ährlund-Richter Andreas A Mirzaie Leila L Farrajota Neves da Silva Pedro P Ursu Ramona G RG Ramqvist Torbjörn T Näsman Anders A

Cancer medicine 20190827 14

<h4>Background</h4>Human papillomavirus (HPV) is a causative agent for tonsillar and base of tongue squamous cell carcinoma (TSCC/BOTSCC), as well as for cervical cancer. Premalignant stages in cervical cancer have been studied extensively, while little is known about premalignant stages in TSCC/BOTSCC and the role of HPV. Here we analyzed differences in gene and protein expression between high-grade dysplasia and invasive cancer in both HPV-positive (HPV<sup>+</sup> ) and HPV-negative (HPV<sup> ...[more]

PMID: 31454186

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Dataset Information

Differences in gene expression between high-grade dysplasia and invasive HPV⁺ and HPV^- tonsillar and base of tongue cancer.

Background

Methods

Results

Conclusions

Publications

Differences in gene expression between high-grade dysplasia and invasive HPV<sup>+</sup> and HPV<sup>-</sup> tonsillar and base of tongue cancer.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

Differences in gene expression between high-grade dysplasia and invasive HPV+ and HPV- tonsillar and base of tongue cancer.

Background

Methods

Results

Conclusions

Publications

Differences in gene expression between high-grade dysplasia and invasive HPV<sup>+</sup> and HPV<sup>-</sup> tonsillar and base of tongue cancer.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Differences in gene expression between high-grade dysplasia and invasive HPV⁺ and HPV^- tonsillar and base of tongue cancer.