Dataset Information

MicroRNA?186?5p mediates osteoblastic differentiation and cell viability by targeting CXCL13 in non?traumatic osteonecrosis.

ABSTRACT: MicroRNAs (miRs) serve varying and important roles in the pathogenesis of non?traumatic osteonecrosis (ON). However, the role miR?186?5p serves in the pathogenesis of osteonecrosis remains unknown and the clinical outcome of ON is still uncertain. The aim of the present study was to determine the expression characteristics, biological function and molecular mechanisms of miR?186?5p, which is associated with cancer development and progression, in osteoblastic differentiation and cell viability. The results of the present study showed that the expression levels of miR?186?5p were significantly higher in clinical non?traumatic ON compared with osteoarthritis samples (P=0.0001). An inverse association was observed between miR?186?5p and CXCL13 expression levels. Furthermore, miR?186?5p inhibited phosphatidylinositol 3 kinase (PI3K)/protein kinase B (AKT) signaling, downregulated osteoblast?specific markers and reduced the viability and differentiation of human mesenchymal stem cells from bone marrow (HMSC?bm) through targeting CXCL13. Increasing expression of CXCL13 in HMSC?bm cells partially restored miR?186?5p?mediated inhibition. In conclusion, abrogation of PI3K/AKT signaling triggered by miR?186?5p/CXCL13 may contribute to ON pathogenesis. These results highlight the possible clinical value of miR?186?5p in treatment for non?traumatic ON.

SUBMITTER: Xu W

PROVIDER: S-EPMC6797973 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

MicroRNA‑186‑5p mediates osteoblastic differentiation and cell viability by targeting CXCL13 in non‑traumatic osteonecrosis.

Xu Weihua W Li Jin J Tian Hongtao H Wang Ruoyu R Feng Yong Y Tang Jing J Jia Jie J

Molecular medicine reports 20190926 5

MicroRNAs (miRs) serve varying and important roles in the pathogenesis of non‑traumatic osteonecrosis (ON). However, the role miR‑186‑5p serves in the pathogenesis of osteonecrosis remains unknown and the clinical outcome of ON is still uncertain. The aim of the present study was to determine the expression characteristics, biological function and molecular mechanisms of miR‑186‑5p, which is associated with cancer development and progression, in osteoblastic differentiation and cell viability. T ...[more]

PMID: 31702033

Similar Datasets

Project description:ObjectiveThe primary aim of the present study was to explore the potential correlation of serum / local CXCL13 expressions and disease severity in non-traumatic osteonecrosis of the femoral head (NT-ONFH).MethodsIn total, NT-ONFH patients (n = 130) together with healthy controls (HCs, n = 130) were included in this investigation. Radiographic progression was evaluated based on the imaging criteria outlined in the ARCO classification system. To assess the diagnostic value of serum CXCL13 in relation to radiographic progression, Receiver operating characteristic (ROC) curve analysis was conducted. Serum CXCL13 levels were quantified utilizing ELISA in all participants. Furthermore, local protein/mRNA expressions of CXCL13 were examined employing immunohistochemistry, western blot, as well as RT-PCR techniques. Clinical severity was appraised using the visual analogue scale (VAS), Harris Hip Score (HHS), and Western Ontario as well as McMaster Universities Osteoarthritis Index (WOMAC).ResultsThe findings revealed a significant reduction in serum CXCL13 levels among NT-ONFH patients in contrast with HCs. Moreover, both mRNA and protein expressions of CXCL13 were markedly decreased in the necrotic area (NA) than the non-necrotic area (NNA) as well as the healthy femoral head tissues. Additionally, serum CXCL13 levels were substantially lower among patients classified as ARCO stage 4 than those at ARCO stage 3. The concentrations of CXCL13 in stage 3 patients were notably diminished relative to those at ARCO stage 2. Notably, serum CXCL13 levels demonstrated a negative association with ARCO grade. Furthermore, these levels were also inversely linked to VAS scores as well as WOMAC scores while displaying a positive association with HHS scores. The findings of ROC curve suggested that reduced serum CXCL13 levels could be an underlying indicator for ARCO stage.ConclusionsThe reduced levels of either serum CXCL13 or local CXCL13 were intricately linked to disease severity for patients with NT-ONFH.

Dataset Information

MicroRNA?186?5p mediates osteoblastic differentiation and cell viability by targeting CXCL13 in non?traumatic osteonecrosis.

Publications

MicroRNA‑186‑5p mediates osteoblastic differentiation and cell viability by targeting CXCL13 in non‑traumatic osteonecrosis.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets