Ontology highlight
ABSTRACT:
SUBMITTER: Chu LA
PROVIDER: S-EPMC6800451 | biostudies-literature | 2019 Oct
REPOSITORIES: biostudies-literature
Nature communications 20191018 1
Optical super-resolution microscopy allows nanoscale imaging of protein molecules in intact biological tissues. However, it is still challenging to perform large volume super-resolution imaging for entire animal organs. Here we develop a single-wavelength Bessel lightsheet method, optimized for refractive-index matching with clarified specimens to overcome the aberrations encountered in imaging thick tissues. Using spontaneous blinking fluorophores to label proteins of interest, we resolve the m ...[more]