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Molecular characterization of the netrin-1 UNC-5 receptor in Lucilia sericata larvae.


ABSTRACT: Larval therapy with Lucilia sericata is a promising strategy in wound healing. Axon guidance molecules play vital roles during the development of the nervous system and also regulate the capacity of neuronal restoration in wound healing. Netrin-1, one of the proteins that larvae secrete, plays a useful role in cell migration and nerve tissue regeneration. The UNC-5 receptor combines with a netrin-1 signal and transmits the signal from one side of the membrane to the other side, initiating a change in cell activity. In the current study, we identified the full length of the UNC-5 receptor mRNA in L. sericata using different sets of primers, including exon junction and specific region primers. The coding sequence (CDS) of the UNC-5 receptor was sequenced and identified to include 633 base-pair nucleic acids, and BLAST analysis on its nucleotide sequence revealed 96% identity with the Lucilia cuprina netrin-1 UNC-5 receptor. The protein residue included 210 amino acids (aa) and coded for a protein with 24 kD weight. This gene lacked the signal peptide. Furthermore, the UPA domain is conserved in UNC-5. It lied at the interval of 26-131 aa. We identified the CDS of netrin-1 UNC-5 receptor in L. sericata. It could be applied to research activities implementing a new essential component design in wound healing.

SUBMITTER: Karamzadeh T 

PROVIDER: S-EPMC6803787 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Molecular characterization of the <i>netrin-1 UNC-5</i> receptor in <i>Lucilia sericata</i> larvae.

Karamzadeh Tahereh T   Alipour Hamzeh H   Shahriari-Namadi Marziae M   Raz Abbasali A   Azizi Kourosh K   Bagheri Masoumeh M   Moemenbellah-Fard Mohammad D MD  

AIMS genetics 20190808 3


Larval therapy with <i>Lucilia sericata</i> is a promising strategy in wound healing. Axon guidance molecules play vital roles during the development of the nervous system and also regulate the capacity of neuronal restoration in wound healing. <i>Netrin-1</i>, one of the proteins that larvae secrete, plays a useful role in cell migration and nerve tissue regeneration. The <i>UNC-5 receptor</i> combines with a <i>netrin-1</i> signal and transmits the signal from one side of the membrane to the o  ...[more]

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