Constrained Mutational Sampling of Amino Acids in HIV-1 Protease Evolution.

ABSTRACT: The evolution of HIV-1 protein sequences should be governed by a combination of factors including nucleotide mutational probabilities, the genetic code, and fitness. The impact of these factors on protein sequence evolution is interdependent, making it challenging to infer the individual contribution of each factor from phylogenetic analyses alone. We investigated the protein sequence evolution of HIV-1 by determining an experimental fitness landscape of all individual amino acid changes in protease. We compared our experimental results to the frequency of protease variants in a publicly available data set of 32,163 sequenced isolates from drug-naïve individuals. The most common amino acids in sequenced isolates supported robust experimental fitness, indicating that the experimental fitnes

SUBMITTER: Boucher JI 

PROVIDER: S-EPMC6804413 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature