Ontology highlight
ABSTRACT:
SUBMITTER: Mizuno K
PROVIDER: S-EPMC6805874 | biostudies-literature | 2019 Oct
REPOSITORIES: biostudies-literature
Mizuno Kei K Akamatsu Shusuke S Sumiyoshi Takayuki T Wong Jing Hao JH Fujita Masashi M Maejima Kazuaki K Nakano Kaoru K Ono Atushi A Aikata Hiroshi H Ueno Masaki M Hayami Shinya S Yamaue Hiroki H Chayama Kazuaki K Inoue Takahiro T Ogawa Osamu O Nakagawa Hidewaki H Fujimoto Akihiro A
Scientific reports 20191022 1
Plasma cell-free DNA (cfDNA) testing plays an increasingly important role in precision medicine for cancer. However, circulating cell-free tumor DNA (ctDNA) is highly diluted by cfDNA from non-cancer cells, complicating ctDNA detection and analysis. To identify low-frequency variants, we developed a program, eVIDENCE, which is a workflow for filtering candidate variants detected by using the ThruPLEX tag-seq (Takara Bio), a commercially-available molecular barcoding kit. We analyzed 27 cfDNA sam ...[more]