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Identification of FosA8, a Plasmid-Encoded Fosfomycin Resistance Determinant from Escherichia coli, and Its Origin in Leclercia adecarboxylata.


ABSTRACT: A plasmid-located fosfomycin resistance gene, fosA8, was identified from a CTX-M-15-producing Escherichia coli isolate recovered from urine. Identification of this gene was obtained by whole-genome sequencing. It encoded FosA8, which shares 79% and 78% amino acid identity with the most closely related FosA2 and FosA1 enzymes, respectively. The fosA8 gene was located on a transferable 50-kb plasmid of IncN type encoding high-level resistance to fosfomycin. In silico analysis and cloning experiments identified fosA8 analogues (99% identity) in the genome of Leclercia decarboxylata, which is an enterobacterial species with natural resistance to fosfomycin. This finding adds L. decarboxylata to the list of enterobacterial species that are a reservoir of fosA-like genes which have been captured from the chromosome of a progenitor and are then acquired by E. coli.

SUBMITTER: Poirel L 

PROVIDER: S-EPMC6811418 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature

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Identification of FosA8, a Plasmid-Encoded Fosfomycin Resistance Determinant from Escherichia coli, and Its Origin in Leclercia adecarboxylata.

Poirel Laurent L   Vuillemin Xavier X   Kieffer Nicolas N   Mueller Linda L   Descombes Marie-Christine MC   Nordmann Patrice P  

Antimicrobial agents and chemotherapy 20191022 11


A plasmid-located fosfomycin resistance gene, <i>fosA8</i>, was identified from a CTX-M-15-producing <i>Escherichia coli</i> isolate recovered from urine. Identification of this gene was obtained by whole-genome sequencing. It encoded FosA8, which shares 79% and 78% amino acid identity with the most closely related FosA2 and FosA1 enzymes, respectively. The <i>fosA8</i> gene was located on a transferable 50-kb plasmid of IncN type encoding high-level resistance to fosfomycin. <i>In silico</i> an  ...[more]

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