Ontology highlight
ABSTRACT:
SUBMITTER: Jiang W
PROVIDER: S-EPMC6811856 | biostudies-literature | 2019 Oct
REPOSITORIES: biostudies-literature
Jiang Wei W Singh Jaideep J Allen Aleique A Li Yue Y Kathiresan Venkatesan V Qureshi Omair O Tangprasertchai Narin N Zhang Xiaojun X Parameshwaran Hari Priya HP Rajan Rakhi R Qin Peter Z PZ
ACS omega 20191009 17
Cas12a (also known as "Cpf1") is a class 2 type V-A CRISPR-associated nuclease that can cleave double-stranded DNA at specific sites. The Cas12a effector enzyme comprises a single protein and a CRISPR-encoded small RNA (crRNA) and has been used for genome editing and manipulation. Work reported here examined in vitro interactions between the Cas12a effector enzyme and DNA duplexes with varying states of base-pairing between the two strands. The data revealed that in the absence of complementarit ...[more]