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Dataset on cytotoxicity effect of polyethylenimine-functionalized graphene oxide nanoparticles on the human embryonic carcinoma stem cell, NTERA2 cell line.

ABSTRACT: The data provided in this article are related to research entitled "Efficiency of graphene oxide nanoparticles as delivery system of SOX2OT siRNA". In this research, the goal is to use PEI-functionalized graphene oxide (PEI-GO) as a carrier for SOX2OTsiRNA delivery. In this article describes how GO coated with PEI and it was tested whether it can be siRNA carrier in NTERA2? Can it absorb siRNA? Whether Go-PEI affects the viability of NTERA2 (NT2: human embryonic carcinoma stem cell), and HeLa cell lines. In this experiment, graphene oxide nanoparticles functionalized with a polycationic polymer, polyethylenimine (PEI). GO-PEI formation was verified with DLS, FTIR tests and zeta sizer. siRNA absorption ability of GO-PEI was tested by gel retardation assay in various weight ratios of GOPEI/siRNA (GOPEI weight/siRNA weight) (w/w ratio). The cell lines were treated with different concentrations of GO-PEI nanoparticles for 24 and 48 hours. Also, the NT2 cells were treated with different concentrations of GO-PEI nanoparticles and PEI for 36 hours. Cytotoxicity of GO-PEI were investigated by calculating the percent of cell survival by MTT assay. MTT data analyzed in excel. Researchers, who want to research on different drugs, could transfer the drug to NT2, HeLa and other cancer cells on GO-PEI (concentration 0 up to 100 mg/L).

SUBMITTER: Sadeghi Z

PROVIDER: S-EPMC6811929 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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Dataset on cytotoxicity effect of polyethylenimine-functionalized graphene oxide nanoparticles on the human embryonic carcinoma stem cell, NTERA2 cell line.

Sadeghi Zahra Z Maleki Parichehr P Mohammadi Bondarkhilli Seyed Abolghasem SA Mohammadi Mehdi M Raheb Jamshid J

Data in brief 20190906

The data provided in this article are related to research entitled "Efficiency of graphene oxide nanoparticles as delivery system of SOX2OT siRNA". In this research, the goal is to use PEI-functionalized graphene oxide (PEI-GO) as a carrier for SOX2OTsiRNA delivery. In this article describes how GO coated with PEI and it was tested whether it can be siRNA carrier in NTERA2? Can it absorb siRNA? Whether Go-PEI affects the viability of NTERA2 (NT2: human embryonic carcinoma stem cell), and HeLa ce ...[more]

PMID: 31667251

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Project description:Graphene and its derivatives are emerging as attractive materials for biomedical applications, including antibacterial, gene delivery, contrast imaging, and anticancer therapy applications. It is of fundamental importance to study the cytotoxicity and biocompatibility of these materials as well as how they interact with the immune system. The present study was conducted to assess the immunotoxicity of graphene oxide (GO) and vanillin-functionalized GO (V-rGO) on THP-1 cells, a human acute monocytic leukemia cell line. The synthesized GO and V-rGO were characterized by using various analytical techniques. Various concentrations of GO and V-rGO showed toxic effects on THP-1 cells such as the loss of cell viability and proliferation in a dose-dependent manner. Cytotoxicity was further demonstrated as an increased level of lactate dehydrogenase (LDH), loss of mitochondrial membrane potential (MMP), decreased level of ATP content, and cell death. Increased levels of reactive oxygen species (ROS) and lipid peroxidation caused redox imbalance in THP-1 cells, leading to increased levels of malondialdehyde (MDA) and decreased levels of anti-oxidants such as glutathione (GSH), glutathione peroxidase (GPX), super oxide dismutase (SOD), and catalase (CAT). Increased generation of ROS and reduced MMP with simultaneous increases in the expression of pro-apoptotic genes and downregulation of anti-apoptotic genes suggest that the mitochondria-mediated pathway is involved in GO and V-rGO-induced apoptosis. Apoptosis was induced consistently with the significant DNA damage caused by increased levels of 8-oxo-dG and upregulation of various key DNA-regulating genes in THP-1 cells, indicating that GO and V-rGO induce cell death through oxidative stress. As a result of these events, GO and V-rGO stimulated the secretion of various cytokines and chemokines, indicating that the graphene materials induced potent inflammatory responses to THP-1 cells. The harshness of V-rGO in all assays tested occurred because of better charge transfer, various carbon to oxygen ratios, and chemical compositions in the rGO. Overall, these findings suggest that it is essential to better understand the parameters governing GO and functionalized GO in immunotoxicity and inflammation. Rational design of safe GO-based formulations for various applications, including nanomedicine, may result in the development of risk management methods for people exposed to graphene and graphene family materials, as these nanoparticles can be used as delivery agents in various biomedical applications.

Project description:BackgroundGraphene and graphene derivative nanoplatelets represent a new generation of nanomaterials with unique physico-chemical properties and high potential for use in composite materials and biomedical devices. To date little is known about the impact graphene nanomaterials may have on human health in the case of accidental or intentional exposure. The objective of this study was to assess the cytotoxic potential of graphene nanoplatelets with different surface chemistry towards a human hepatoma cell line, Hep G2, and identify the underlying toxicity targets.MethodsGraphene oxide (GO) and carboxyl graphene (CXYG) nanoplatelet suspensions were obtained in water and culture medium. Size frequency distribution of the suspensions was determined by means of dynamic light scattering. Height, lateral dimension and shape of the nanoplatelets were determined using atomic force and electron microscopy. Cytotoxicity of GO and CXYG nanoplatelets was assessed in Hep G2 cells using a battery of assays covering different modes of action including alterations of metabolic activity, plasma membrane integrity and lysosomal function. Induction of oxidative stress was assessed by measuring intracellular reactive oxygen species levels. Interaction with the plasma membrane, internalization and intracellular fate of GO and CXYG nanoplatelets was studied by scanning and transmission electron microscopy.ResultsSupplementing culture medium with serum was essential to obtain stable GO and CXYG suspensions. Both graphene derivatives had high affinity for the plasma membrane and caused structural damage of the latter at concentrations as low as 4 μg/ml. The nanoplatelets penetrated through the membrane into the cytosol, where they were concentrated and enclosed in vesicles. GO and CXYG accumulation in the cytosol was accompanied by an increase in intracellular reactive oxygen species (ROS) levels, alterations in cellular ultrastructure and changes in metabolic activity.ConclusionsGO and CXYG nanoplatelets caused dose- and time-dependent cytotoxicity in Hep G2 cells with plasma membrane damage and induction of oxidative stress being important modes of toxicity. Both graphene derivatives were internalized by Hep G2, a non-phagocytotic cell line. Moreover, they exerted no toxicity when applied at very low concentrations (< 4 μg/ml). GO and CXYG nanoplatelets may therefore represent an attractive material for biomedical applications.

Dataset Information

Dataset on cytotoxicity effect of polyethylenimine-functionalized graphene oxide nanoparticles on the human embryonic carcinoma stem cell, NTERA2 cell line.

Publications

Dataset on cytotoxicity effect of polyethylenimine-functionalized graphene oxide nanoparticles on the human embryonic carcinoma stem cell, NTERA2 cell line.

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