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Water-deficit responsive microRNAs in the primary root growth zone of maize.

ABSTRACT: BACKGROUND:MicroRNA-mediated gene regulatory networks play a significant role in plant growth and development and environmental stress responses. RESULTS:We identified 79 microRNAs (miRNAs) and multiple miRNA variants (isomiRs) belonging to 26 miRNA families in the primary root growth zone of maize seedlings grown at one of three water potentials: well-watered (-?0.02?MPa), mild water deficit stress (-?0.3?MPa), and severe water deficit stress (-?1.6?MPa). The abundances of 3 miRNAs (mild stress) and 34 miRNAs representing 17 families (severe stress) were significantly different in water-deficit stressed relative to well-watered controls (FDR?

SUBMITTER: Seeve CM

PROVIDER: S-EPMC6814125 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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Water-deficit responsive microRNAs in the primary root growth zone of maize.

Seeve Candace M CM Sunkar Ramanjulu R Zheng Yun Y Liu Li L Liu Zhijie Z McMullen Michael M Nelson Sven S Sharp Robert E RE Oliver Melvin J MJ

BMC plant biology 20191024 1

<h4>Background</h4>MicroRNA-mediated gene regulatory networks play a significant role in plant growth and development and environmental stress responses.<h4>Results</h4>We identified 79 microRNAs (miRNAs) and multiple miRNA variants (isomiRs) belonging to 26 miRNA families in the primary root growth zone of maize seedlings grown at one of three water potentials: well-watered (- 0.02 MPa), mild water deficit stress (- 0.3 MPa), and severe water deficit stress (- 1.6 MPa). The abundances of 3 miRN ...[more]

PMID: 31651253

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Project description:Reactive oxygen species (ROS) can act as signaling molecules involved in the acclimation of plants to various abiotic and biotic stresses. However, it is not clear how the generalized increases in ROS and downstream signaling events that occur in response to stressful conditions are coordinated to modify plant growth and development. Previous studies of maize (Zea mays L.) primary root growth under water deficit stress showed that cell elongation is maintained in the apical region of the growth zone but progressively inhibited further from the apex, and that the rate of cell production is also decreased. It was observed that apoplastic ROS, particularly hydrogen peroxide (H2O2), increased specifically in the apical region of the growth zone under water stress, resulting at least partly from increased oxalate oxidase activity in this region. To assess the function of the increase in apoplastic H2O2 in root growth regulation, transgenic maize lines constitutively expressing a wheat oxalate oxidase were utilized in combination with kinematic growth analysis to examine effects of increased apoplastic H2O2 on the spatial pattern of cell elongation and on cell production in well-watered and water-stressed roots. Effects of H2O2 removal (via scavenger pretreatment) specifically from the apical region of the growth zone were also assessed. The results show that apoplastic H2O2 positively modulates cell production and root elongation under well-watered conditions, whereas the normal increase in apoplastic H2O2 in water-stressed roots is causally related to down-regulation of cell production and root growth inhibition. The effects on cell production were accompanied by changes in spatial profiles of cell elongation and in the length of the growth zone. However, effects on overall cell elongation, as reflected in final cell lengths, were minor. These results reveal a fundamental role of apoplastic H2O2 in regulating cell production and root elongation in both well-watered and water-stressed conditions.

Dataset Information

Water-deficit responsive microRNAs in the primary root growth zone of maize.

Publications

Water-deficit responsive microRNAs in the primary root growth zone of maize.

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