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ROS-based lethality of Caenorhabditis elegans mitochondrial electron transport mutants grown on Escherichia coli siderophore iron release mutants.


ABSTRACT: Caenorhabditis elegans consumes bacteria, which can supply essential vitamins and cofactors, especially for mitochondrial functions that have a bacterial ancestry. Therefore, we screened the Keio Escherichia coli knockout library for mutations that induce the C. elegans hsp-6 mitochondrial damage response gene, and identified 45 E. coli mutations that induce hsp-6::gfp We tested whether any of these E. coli mutations that stress the C. elegans mitochondrion genetically interact with C. elegans mutations in mitochondrial functions. Surprisingly, 4 E. coli mutations that disrupt the import or removal of iron from the bacterial siderophore enterobactin were lethal in combination with a collection of C. elegans mutations that disrupt particular iron-sulfur proteins of the electron transport chain. Bacterial mutations that fail to synthesize enterobactin are not synthetic lethal with these C. elegans mitochondrial mutants; it is the enterobactin-iron complex that is lethal in combination with the C. elegans mitochondrial mutations. Antioxidants suppress this inviability, suggesting that reactive oxygen species (ROS) are produced by the mutant mitochondria in combination with the bacterial enterobactin-iron complex.

SUBMITTER: Govindan JA 

PROVIDER: S-EPMC6815122 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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ROS-based lethality of <i>Caenorhabditis elegans</i> mitochondrial electron transport mutants grown on <i>Escherichia coli</i> siderophore iron release mutants.

Govindan J Amaranath JA   Jayamani Elamparithi E   Ruvkun Gary G  

Proceedings of the National Academy of Sciences of the United States of America 20191007 43


<i>Caenorhabditis elegans</i> consumes bacteria, which can supply essential vitamins and cofactors, especially for mitochondrial functions that have a bacterial ancestry. Therefore, we screened the Keio <i>Escherichia coli</i> knockout library for mutations that induce the <i>C. elegans hsp-6</i> mitochondrial damage response gene, and identified 45 <i>E. coli</i> mutations that induce <i>hsp-6::gfp</i> We tested whether any of these <i>E. coli</i> mutations that stress the <i>C. elegans</i> mit  ...[more]

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