Dataset Information

Urinary fungi associated with urinary symptom severity among women with interstitial cystitis/bladder pain syndrome (IC/BPS).

ABSTRACT:

Purpose

To correlate the presence of fungi with symptom flares, pain and urinary severity in a prospective, longitudinal study of women with IC/BPS enrolled in the MAPP Research Network.

Methods

Flare status, pelvic pain, urinary severity, and midstream urine were collected at baseline, 6 and 12 months from female IC/BPS participants with at least one flare and age-matched participants with no reported flares. Multilocus PCR coupled with electrospray ionization/mass spectrometry was used for identification of fungal species and genus. Associations between "mycobiome" (species/genus presence, relative abundance, Shannon's/Chao1 diversity indices) and current flare status, pain, urinary severity were evaluated using generalized linear mixed models, permutational multivariate analysis of variance, Wilcoxon's rank-sum test.

Results

The most specific analysis detected 13 fungal species from 8 genera in 504 urine samples from 202 females. A more sensitive analysis detected 43 genera. No overall differences were observed in fungal species/genus composition or diversity by flare status or pain severity. Longitudinal analyses suggested greater fungal diversity (Chao1 Mean Ratio 3.8, 95% CI 1.3-11.2, p?=?0.02) and a significantly greater likelihood of detecting any fungal species (OR?=?5.26, 95% CI 1.1-25.8, p?=?0.04) in high vs low urinary severity participants. Individual taxa analysis showed a trend toward increased presence and relative abundance of Candida (OR?=?6.63, 95% CI 0.8-58.5, p?=?0.088) and Malassezia (only identified in 'high' urinary severity phenotype) for high vs low urinary symptoms.

Conclusion

This analysis suggests the possibility that greater urinary symptom severity is associated with the urinary mycobiome urine in some females with IC/BPS.

SUBMITTER: Nickel JC

PROVIDER: S-EPMC6815247 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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Publications

Urinary fungi associated with urinary symptom severity among women with interstitial cystitis/bladder pain syndrome (IC/BPS).

Nickel J Curtis JC Stephens Alisa A Landis J Richard JR Mullins Chris C van Bokhoven Adrie A Anger Jennifer T JT Ackerman A Lenore AL Kim Jayoung J Sutcliffe Siobhan S Krol Jaroslaw E JE Sen Bhaswati B Hammond Jocelyn J Ehrlich Garth D GD

World journal of urology 20190426 2

<h4>Purpose</h4>To correlate the presence of fungi with symptom flares, pain and urinary severity in a prospective, longitudinal study of women with IC/BPS enrolled in the MAPP Research Network.<h4>Methods</h4>Flare status, pelvic pain, urinary severity, and midstream urine were collected at baseline, 6 and 12 months from female IC/BPS participants with at least one flare and age-matched participants with no reported flares. Multilocus PCR coupled with electrospray ionization/mass spectrometry w ...[more]

PMID: 31028455

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Dataset Information

Urinary fungi associated with urinary symptom severity among women with interstitial cystitis/bladder pain syndrome (IC/BPS).

Purpose

Methods

Results

Conclusion

Publications

Urinary fungi associated with urinary symptom severity among women with interstitial cystitis/bladder pain syndrome (IC/BPS).

Similar Datasets

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