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RaGOO: fast and accurate reference-guided scaffolding of draft genomes.


ABSTRACT: We present RaGOO, a reference-guided contig ordering and orienting tool that leverages the speed and sensitivity of Minimap2 to accurately achieve chromosome-scale assemblies in minutes. After the pseudomolecules are constructed, RaGOO identifies structural variants, including those spanning sequencing gaps. We show that RaGOO accurately orders and orients 3 de novo tomato genome assemblies, including the widely used M82 reference cultivar. We then demonstrate the scalability and utility of RaGOO with a pan-genome analysis of 103 Arabidopsis thaliana accessions by examining the structural variants detected in the newly assembled pseudomolecules. RaGOO is available open source at https://github.com/malonge/RaGOO .

SUBMITTER: Alonge M 

PROVIDER: S-EPMC6816165 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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RaGOO: fast and accurate reference-guided scaffolding of draft genomes.

Alonge Michael M   Soyk Sebastian S   Ramakrishnan Srividya S   Wang Xingang X   Goodwin Sara S   Sedlazeck Fritz J FJ   Lippman Zachary B ZB   Schatz Michael C MC  

Genome biology 20191028 1


We present RaGOO, a reference-guided contig ordering and orienting tool that leverages the speed and sensitivity of Minimap2 to accurately achieve chromosome-scale assemblies in minutes. After the pseudomolecules are constructed, RaGOO identifies structural variants, including those spanning sequencing gaps. We show that RaGOO accurately orders and orients 3 de novo tomato genome assemblies, including the widely used M82 reference cultivar. We then demonstrate the scalability and utility of RaGO  ...[more]

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