Dataset Information

Automated analysis for differentiating leukocytes in body fluids using the software "biological liquid application" on ADVIA2120/2120i hematology analyzer.

ABSTRACT:

Introduction

We evaluated the "Biological liquid application ADVIA2120" software for differentiating the percentage of polymorphonucleated (%PMN) and mononucleated cells (%MN) in ascitic, pleural, and peritoneal dialysis (PD) fluid.

Methods

Biological fluid test results of 193 specimens obtained by automated methods (87 with and 106 without dedicated software) were compared with May-Grünwald-Giemsa (MGG) stained blood smears. Limit of detection (LoD) and quantitation (LoQ), repeatability, and inaccuracy were assessed.

Results

Good agreement between the automated methods with dedicated software and the manual method for %PMN and %MN was obtained for leukocyte differentiation in ascitic and pleural fluids, while correlation with the manual method for PD fluid was poor, both with and without the dedicated software.

Conclusions

We demonstrated that the automated differentiation of leukocytes with dedicated software on the ADVIA2120 analyzer for body fluids is a good alternative to the microscopic reference method for peritoneal and pleural specimens, but not for PD fluids.

SUBMITTER: Falda A

PROVIDER: S-EPMC6817033 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

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Publications

Automated analysis for differentiating leukocytes in body fluids using the software "biological liquid application" on ADVIA2120/2120i hematology analyzer.

Falda Alessandra A Doretto Paolo P

Journal of clinical laboratory analysis 20180525 8

<h4>Introduction</h4>We evaluated the "Biological liquid application ADVIA2120" software for differentiating the percentage of polymorphonucleated (%PMN) and mononucleated cells (%MN) in ascitic, pleural, and peritoneal dialysis (PD) fluid.<h4>Methods</h4>Biological fluid test results of 193 specimens obtained by automated methods (87 with and 106 without dedicated software) were compared with May-Grünwald-Giemsa (MGG) stained blood smears. Limit of detection (LoD) and quantitation (LoQ), repeat ...[more]

PMID: 29802648

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Dataset Information

Automated analysis for differentiating leukocytes in body fluids using the software "biological liquid application" on ADVIA2120/2120i hematology analyzer.

Introduction

Methods

Results

Conclusions

Publications

Automated analysis for differentiating leukocytes in body fluids using the software "biological liquid application" on ADVIA2120/2120i hematology analyzer.

Similar Datasets

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