A rapid and simple liquid chromatography-tandem mass spectrometry method for the measurement of testosterone, androstenedione, and dehydroepiandrosterone in human serum.

ABSTRACT: The assay of androgens plays an important role for the differential diagnosis of androgen-related endocrine diseases. We developed a rapid and simple liquid chromatography tandem mass spectrometry (LC-MS/MS) method with small sample volume and simple sample preparation for simultaneous determination of serum testosterone, androstenedione and dehydroepiandrosterone (DHEA) in adults. We spiked 100 μL of serum with 200 μL of internal standard solution, which was prepared in methanol, for matrix effect relief and protein precipitation. The obtained supernatant was used directly for LC-MS/MS analysis. The validated method exhibits excellent linearity for each analyte with linear correlation coefficient above 0.992. The total precisions at three concentrations were 15.66%, 8.81% and 4.34% for te

SUBMITTER: Xu W 

PROVIDER: S-EPMC6817279 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature